A kind of bioadhesive with high adhesive strength and preparation method thereof
A bio-adhesive and adhesive strength technology, applied in the field of bioengineering, can solve the problems of restricting the application of adhesives, toxic and side effects, etc., and achieve the effects of green environmental protection, low cost, and improved adhesion performance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0046] The present invention also provides a method for preparing a bioadhesive with high adhesive strength, comprising the following steps:
[0047] A) dissolving the supercharged protein and the surfactant in ultrapure water respectively to obtain a supercharged protein solution and a surfactant solution;
[0048] The surfactant is a cationic surfactant or an anionic azobenzene surfactant;
[0049] The cationic surfactant has a catechol structural unit;
[0050] B) adding the surfactant solution dropwise into the supercharged protein solution, and mixing uniformly to obtain a binder solution;
[0051] C) centrifuging the adhesive solution and then freeze-drying for 0.2-0.5 hours to obtain a bioadhesive with high adhesive strength.
[0052]In the present invention, the kind, dosage and the supercharged protein, cationic surfactant and anionic azobenzene surfactant are derived from the above-mentioned supercharged protein, cationic surfactant and anionic The type, dosage an...
Embodiment 2
[0064] The bioadhesive (K108-NAT) was prepared according to the method in Example 1, except that the charge number of the super positively charged protein in this example was 108.
[0065] The bioadhesive in the present embodiment is smeared on the bottom of two 20mL glass vials, it is bonded together, carry out weighing test, the result is as follows: figure 2 As shown, there are figure 2 It can be seen that the bioadhesive used in this embodiment can bear a weight of 600g when used on the peeling surface.
Embodiment 3
[0067] Transform the super negatively charged protein E series vector plasmids into the E. coli expression strain BL21DE3, pick a single clone colony, and culture overnight with LB culture medium; add the overnight activated expression seed liquid to TB medium, and wait until the bacterial liquid reaches the OD600 value When it was 0.8, the inducer isopropyl-β-D-thiogalactopyranoside was added and the temperature was lowered to 30 degrees for overexpression. Induce 12 hours to collect the bacteria, resuspend with lysis buffer, add protease inhibitors, DNase, lysozyme, break the bacteria with a high-pressure crusher, centrifuge at a speed above 10,000 rpm, collect the supernatant, and purify by HPLC to finally obtain super negative Charge protein E. In this implementation case, the number of charges per molecule of the E series is 36.
[0068] An aqueous solution of unfolded negatively charged protein with a concentration of 220 μmol / L and an aqueous solution of cationic surfa...
PUM
| Property | Measurement | Unit |
|---|---|---|
| water content | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More