Method for obtaining quickly-growing carassius through restraining mstn gene expression of muscular tissue

Abstract

The invention belongs to the technical field of gene expression, and discloses a method for obtaining quickly-growing carassius through restraining mstn gene expression of muscular tissue. The methodcomprises the steps of constructing a transgenic vector, combining a muscular tissue specific expression promoter with a csy4 system in the transgenic vector, and inserting a reverse DNA sequence of atarget gene at the lower course of the promoter; after a transgenic element is integrated into a host genome, performing transcription to produce two RNA fragments which are complementary with different locations of mRNA of the target gene, recognizing a formed antisense-RNA / mRNA dimer by an endogenous nuclease, and performing cutting and degradation so that the mRNA level of the target gene is reduced and desired some phenotypes or biochemical characters are obtained. The experiment indicates that the two antisense RNA can lead to notable reduction of the mRNA level of mstn of an experimental group; sampling is performed after membrane appearance, 10 fishes are weighed each time, and the average weight is taken; and a control group and the experimental group are compared after 20 days ofmembrane appearance, in the experimental group, fish individuals are robust, and the body length and the body weight of the fishes in the experimental group are notably larger than those of the fishes in the control group.

Description

technical field [0001] The invention belongs to the technical field of gene expression, and in particular relates to a method for obtaining fast-growing crucian carp by inhibiting the expression of mstn gene in muscle tissue. Background technique [0002] Currently, technologies for inhibiting endogenous gene expression mainly include MO technology, RNAi technology and CRISPR / Cas9 gene editing technology. MO technology is an antisense technology. Its principle is to artificially replace the five-carbon sugar ring in nucleotides with a morphine ring, and at the same time change the phosphate group to obtain a DNA molecular analog. Drugs can bind to RNA in the form of complementary base pairing, thereby preventing the combination of ribosomes and RNA, so that the expression level of genes is blocked. The inhibitory effect of MO technology is obvious, but it can only be introduced into fertilized eggs or cells through in vitro synthesis, and temporarily (valid for about 4 days...

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Problems solved by technology

[0005] To sum up, the problem in the prior art is that the research of this gene in crucian crucian carp is less at present.

[0011] The significance of solving the above-mentioned technical problems: Although the green crucian carp has multiple excellent characters such as high nutritional value and strong stress resistance, compared with the four major family carps, the growth rate is slow, which is the main factor affecting its breeding benefits. Therefore, to improve the growth of green crucian carp Speed, can greatly improve the efficiency of crucian carp breeding

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