Rapid culture propagation method of locus root toxin-free seedling

Abstract

The invention discloses a rapid culture propagation method of a locus root toxin-free seedling, comprising: taking the stem tip of lotus root as an explant, peeling off an outer sheath of the stem tip, inoculating in an MS or B5 medium in which 6- benzylamino adenine or ZRs and naphthylacetic acid are added; after culturing for 60 to 80 days, taking a part, with a node, of a test tube seedling forsubculture; rapid subculture propagation: transferring the test tube seedling to an MS or B5 medium in which 6- benzylamino adenine, naphthylacetic acid and sugar are added, so that a lot of test tube seedlings can be formed after 30-60 d; rooting culture: transferring the test tube seedlings to an MS or B5 medium in which 6- benzylamino adenine, naphthylacetic acid and sugar are added, so thata lot of roots can be formed after 20-50 d, and the transplanting field survival rate of the culture seedlings reaches 80% or above.The method uses the lotus root stem tip as the xplant to establisha lotus root detoxification rapid propagation system with a propagation coefficient of 8-20, which can obtain a large number of virus-free seedlings without variation, the virus-free seedlings have the transplanting survival rate of 80% or above.and strong growth potential, the quantity of seeds used can be greatly reduced, and the seedling transportation is facilitated, thereby solving the problems in the prior art.

Description

technical field [0001] The invention relates to the technical field of vegetable seedling production, in particular to a method for rapidly cultivating and propagating lotus root virus-free seedlings. Background technique [0002] Lotus root is a traditional characteristic aquatic vegetable in southern my country. It has good nutritional and health functions, and it is also the main foreign exchange earning vegetable in my country. Accumulation of virus due to long-term use of asexual reproduction. At present, serious occurrences have occurred in all production areas, and the resulting "stiff lotus root" problem has caused serious degradation of the species, rhizomes have become smaller, stiffer, and the quality has deteriorated, and the yield has declined severely, which has affected its export earnings and its domestic market. competitiveness. Efforts to improve the quality and yield of bulbs, increase the reproduction coefficient, and solve the problem of "stiff lotus r...

AI Technical Summary

Problems solved by technology

At present, the method of strict field selection every year is generally used to improve or alleviate the occurrence of the above problems, but the labor intensity is high, the technical requirements are high, the effect is not significant, and the above practical problems cannot really be completely solved.