Rapid breeding method of green tea sprout
A technology for mountain green tea and seedlings, applied in the field of plant tissue culture, can solve the problems of long breeding period, low survival rate, low germination rate, etc., and achieve the effects of maintaining excellent characters, high transplanting survival rate, and stable genetic characteristics.
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Embodiment 1
[0025] (1) Selection and sterilization of explants
[0026] Take the ripe berries of camellia green tea, soak them in 0.05% detergent solution for 10 minutes, then rinse them with running water for 15 minutes, and then wash them with 0.1% HgCl 2 Disinfect the solution for 10 minutes, then use tweezers to peel off the pericarp and take the seeds to inoculate in the seed germination medium for germination culture. The temperature of the culture room is 24-26°C, and the seeds are sterilized by this method without pollution;
[0027] Seed germination medium is MS+1.5mg·L -1 6-BA+0.5mg·L -1 NAA+sucrose 25g·L -1 +Agar 5g·L -1 , pH6.0.
[0028] (2) Induction of the first generation
[0029] The seeds began to germinate on 20 days (the germination rate was 83.2%). After the cotyledons unfolded, the root system of the aseptic seedlings was cut off and transferred to the cluster bud induction medium for cultivation. The cultivation was carried out for 10 days (temperature 24-26°C, ...
Embodiment 2
[0040] Others are basically the same as in Example 1, only with 0.1%HgCl in step (1) 2 After the solution was disinfected for 5 minutes, the contamination rate was 16.7%, the germination rate was 83.4%, the induction rate was 98.7%, the multiplication factor was 12.7, the rooting rate was 92%, and the transplanting survival rate was 85.8%.
Embodiment 3
[0042] Others are basically the same as in Example 1, only the seed germination medium in step (1) is MS+0.5mg L -1 6-BA+1.0mg·L -1 NAA+sucrose 30g·L -1 +Agar 5g·L -1 , pH 6.0; the temperature of the culture room was 26-28°C, and the results began to germinate after 20 days, the germination rate was 84.6%, the induction rate was 98.5%, the multiplication factor was 12.5, the rooting rate was 91%, and the transplanting survival rate was 86.1%.
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