Induced liquid and preparation method thereof and hirudin/lumbrokinase extraction method and hirudin lumbrokinase oral liquid and preparation method and application thereof

A lumbrokinase oral liquid and a technology for an extraction method, which are applied in the extraction and application fields of hirudin and lumbrokinase, can solve the problem of not being able to promote leeches to spit out saliva and the like, and achieve the improvement of antithrombotic efficacy, good human safety, and maintenance of human health. Effect

Pending Publication Date: 2019-11-22
王世生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such an induction solution is only suitable for extracting hirudin from leeches, but not for extracting lumbrokinase from earthworms, and its function is to promote leeches to secrete saliva, but not to promote leeches to spit out saliva

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Present embodiment finally will prepare a kind of preparation method of hirudin lumbrokinase oral liquid, specifically is divided into following steps:

[0050] (1) Preparation of induction solution

[0051] The raw materials of the induction solution are prepared from natural and commonly used ingredients chili and ginger (cut chili and ginger into sections), add 1000ml of water to boil on high heat, add 1000ml of water to boil on high heat, then boil on low heat for 30 minutes, turn off the heat and let it cool, then use 80 mesh Remove the residue by filtering through a filter, then add physiological saline at a ratio of 1:1, adjust the pH value to 6.5-7.5, and prepare the induced solution for later use. Among them, the high fire and small fire refer to the relevant regulations or experience in the field of traditional Chinese medicine.

[0052] (2) Extracting hirudin and lumbrokinase from living leeches and earthworms

[0053] (1) Select healthy and mature blood-su...

Embodiment 2

[0065] A method for preparing hirudin lumbrokinase oral liquid. The difference from Example 1 is that the weight ratio of blood-sucking leeches and Eisenia chinensis in step (2) is 3:1.

Embodiment 3

[0067] A preparation method of hirudin lumbrokinase oral liquid, the difference from Example 1 is that in step (2), the weight ratio of blood-sucking leeches and Eisenia chinensis is 1:1.

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PUM

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Abstract

The invention relates to an induced liquid and a preparation method thereof. The induced liquid is prepared by using irritating ingredients. The induced liquid can promote leeches and / or earthworms tosecrete and spit out hirudin and / or lumbrokinase. The invention also provides a method for extracting hirudin and / or lumbrokinase. The induced liquid is used to extract hirudin and / or lumbrokinase from living leeches and / or earthworms. The yield of hirudin and / or lumbrokinase is high, and the active content of hirudin and lumbrokinase is retained to the maximum. The invention also provides a hirudin lumbrokinase oral liquid and a preparation method and application thereof. The induced liquid is used to induce living blood-sucking leeches to secrete and spit out hirudin and eisenia fetida to secrete and spit out lumbrokinase and to preserve other medicinal ingredients in the leeches and earthworms. The disadvantage that hirudin and lumbrokinase contain a large amount of impurities and theimpurities are difficult to remove by filtration is overcome. In addition, the induced liquid has no fishy odor, and the active content and drug properties of hirudin and lumbrokinase are retained tothe maximum. The method is simple and easy to operate, and the antithrombotic and thrombolytic effects are obviously improved.

Description

technical field [0001] The invention belongs to the field of extraction and application of hirudin and lumbrokinase, in particular, relates to an induction solution and a preparation method thereof; the invention also relates to a method for extracting hirudin and / or lumbrokinase; the invention also relates to hirudin and lumbrokinase oral liquid And its preparation method and application. Background technique [0002] Leeches, commonly known as leeches, are non-toxic after modern research. It is a traditional Chinese medicine in my country, which was first recorded in "Shen Nong's Herbal Classic". It has high medicinal value and is effective in treating stroke, hypertension, dredging, amenorrhea, bruises and other diseases. [0003] Hirudin is the most active ingredient among various active ingredients extracted from leeches and their salivary glands. It is a small molecular protein "polypeptide" composed of 65-66 amino acids. Hirudin is rich in hirudin. Hirudin has a str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/815C12N9/68A61K38/58A61K38/48A61K9/08A61P7/02A61P13/12A61P25/02A61P29/00A61P9/14A61P35/00
CPCA61K9/0095A61K38/48A61K38/58A61K47/46A61P7/02A61P9/14A61P13/12A61P25/02A61P29/00A61P35/00C07K14/815C12N9/6408C12Y304/21007
Inventor 王世生
Owner 王世生
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