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Method for detecting tetracycline antibiotic residues in eggs based on SERS

A technology of tetracyclines and antibiotics, which is applied in the preparation of test samples, measuring devices, instruments, etc., can solve the problems of time-consuming detection, low sensitivity, and unsatisfactory separation system efficiency, and achieve the effect of easy real-time detection.

Active Publication Date: 2019-12-03
山东恒诚检测科技有限公司
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Problems solved by technology

However, microbial methods and enzyme-linked detection and immunological techniques are cumbersome, low-sensitivity, and time-consuming (>2h); accurate detection generally uses GB / T 5009.116-2003 Determination of Oxytetracycline, Tetracycline, and Chlortetracycline Residues in Livestock and Poultry Meat (high performance liquid chromatography) method, but it needs to use large-scale instruments such as liquid chromatography, which is not suitable for rapid detection, and the detection is time-consuming; at the same time, the efficiency of the separation system for complex multi-component systems is not ideal, and due to a large number of The use of organic solvents is easy to cause pollution to the environment

Method used

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  • Method for detecting tetracycline antibiotic residues in eggs based on SERS
  • Method for detecting tetracycline antibiotic residues in eggs based on SERS
  • Method for detecting tetracycline antibiotic residues in eggs based on SERS

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Embodiment Construction

[0026] (1) Preparation of activity-enhanced substrate

[0027] Adopt traditional microwave heating method to prepare silver nano sol particle, promptly under microwave heating condition, utilize trisodium citrate to reduce silver nitrate to prepare silver sol, after reacting for a period of time, when the color of silver nano sol changes from red to gray-green, Stop heating and take out the silver nano sol, cool down to room temperature naturally, wash with ethanol at 5000r / min for at least 3 times, and store at 4°C for later use; absorb 10 μL of silver nano sol and evenly attach it to the carbon working electrode of the screen printing electrode, and put it in the oven Dry at 60°C, repeat the plating three times, and make a 0.5 cm×0.5 cm silver nano-coating as a SERS activity-enhancing substrate;

[0028] (2) Preparation of egg extract samples

[0029] Take eggs and break their shells, put them in a homogenizer to fully function evenly, take 5g of homogeneous egg liquid and ...

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Abstract

The invention relates to the technical field of food detection, in particular to a method for detecting tetracycline antibiotic residues in eggs based on SERS (surface enhanced raman scattering). Themethod comprises the following steps: extracting an egg acetone extracting solution by using silver nano sol particles as an activity enhancing substrate and carrying out electrostatic enrichment andRaman spectrum detection to obtain an SERS spectrum; and carrying out comparison on working standard curve points with different concentrations of the acetone egg extracting solution without tetracyclines and an acetone extracting solution containing antibiotics, finding out a Raman optimal characteristic peak position as a quantitative peak. According to the invention, the method having advantages of simpleness and rapidness is easy to carry out real-time detection and carry out quantitative analysis and thus has the important significance in strict control of antibiotic pollution in food.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for detecting tetracycline antibiotic residues in eggs based on SERS. Background technique [0002] my country is a large poultry breeding country. In 2005, the total annual output of poultry eggs reached 287.95 million tons, ranking first in the world. Eggs are very popular among consumers because of their rich nutrition, easy absorption, good taste and low price. Eggs account for the vast majority of the poultry egg market, and more than 90% of eggs are produced by farmers and small businesses. Although the current therapeutic drugs allowed for raising non-pollution food layer hens are strictly limited, in actual production, in order to reduce the disease The risk of abuse and misuse of antibiotics is very serious, and the problem of drug residues is also increasing through the effects of the environment and the food chain. [0003] Tetracycline, oxytetracyclin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/34
CPCG01N1/34G01N21/658
Inventor 苑旭洲施建刚李晓燕
Owner 山东恒诚检测科技有限公司
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