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Human y-str gene locus fluorescent labeling kit and detection method

A technology of Y-STR and fluorescent labeling, which is applied in the fields of biochemical equipment and methods, and microbial determination/inspection, etc. It can solve the problems of limited understanding of Y-STR genetic polymorphisms, and the ability to identify and identify relatives needs to be improved. Achieve the effect of improving individual recognition ability and polymorphism

Active Publication Date: 2020-07-03
湖北崇新司法鉴定中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to the main 35 Y-STR loci, the understanding of Y-STR genetic polymorphisms is limited, and the ability of regional individual identification and kinship identification also needs to be improved

Method used

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  • Human y-str gene locus fluorescent labeling kit and detection method
  • Human y-str gene locus fluorescent labeling kit and detection method
  • Human y-str gene locus fluorescent labeling kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Paternity Test

[0024] 1. Sample source

[0025] The sample in this example is human venous blood, provided by a judicial identification center. Genomic DNA was extracted using the method in "GA / T 383-2014 Forensic Science DNA Laboratory Test Specification".

[0026] 2. Amplification detection

[0027] The Hyersch STRtyper-21G kit was used to detect the typing of 21 human autosomal STRs, and the kit of the present invention was used to detect the typing of 17 Y-STRs. The results of typing comparison between father and child are shown in Table 2: 13 of the 21 autosomal STR test results of father and son do not conform to the genetic law, and father-son relationship can be ruled out. Further detection of Y-chromosomal STR revealed that 12 of the 17 Y-STR loci did not conform to the genetic law, which also verified the above conclusion.

[0028]

[0029]

Embodiment 2

[0030] Embodiment 2: Crowd frequency investigation database construction

[0031] 1. Collect male specimens from different regions

[0032] In this experiment, 1260 samples from different regions were: 394 cases in Taiyuan City (Shanxi Province), 162 cases in Chongqing City, 154 cases in Ulanqab City (Inner Mongolia Autonomous Region), 155 cases in Sanmenxia City (Henan Province), and 155 cases in Foshan City ( Guangdong Province) 95 cases, Hainan Li nationality 113 cases, Hainan Miao nationality 63 cases, Jingzhou City (Hubei Province) 124 cases.

[0033] 2. Genomic DNA extraction

[0034] The extraction of DNA was carried out according to "GA / T 383-2014 Forensic Science DNA Laboratory Inspection Specification".

[0035] 3. Using 17 pairs of Y-STR loci-specific primers to construct a multiplex PCR amplification system

[0036]The PCR reaction is a 15 μL system, including: 7.5 μL PCR Master Mix, 1.5 μL PCR upstream and downstream primer mixture (primer final concentration r...

Embodiment 3

[0047] Embodiment 3: species-specific research

[0048] 1. Collection of animal samples

[0049] The animal samples in this experiment were from chicken, cow, fish, pig, rabbit, mouse, sheep and shrimp. Genomic DNA was extracted using the Qiagen (DNeasyBlood & Tissue Kit) kit.

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Abstract

The invention discloses a human Y-STR (Y-short tandem repeat) locus fluorescent labeled kit and a detection method. The kit contains four-color fluorescent labeled 17 specific primer pairs, PCR MasterMix, fluorescent labeled M13 universal primers, ddH2O, deionized formamide and a SIZE-500 Plus interior label, and 17 new Y-STR loci can be amplified simultaneously and include DYS715, DYS709, DYS716, DYS713, DYS607, DYS718, DYS723, DYS708, DYS714, DYS712, DYS717, DYS721, DYS605, DYS719, DYS726, DYS598 and DYS722. The kit has no coincident loci with a current Y-STR kit, and can be applied to identification of difficult relatives and identification of family screening of criminal suspects.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a Y-STR gene locus kit and a detection method for database construction and investigation, paternity identification and DNA family tree construction. Background technique [0002] Y chromosome STR genetic marker refers to the short tandem repeats (shorttandem repeats, STR) existing in the non-recombination region of human Y chromosome, most of which contain two or more different repeat units, the core repeat sequence or repeat number of individuals in the population Different, forming population genetic polymorphism. It has three characteristics of male-specific, paternal inheritance and haplotype inheritance. The Y-STR test technology is a supplement to the autosomal STR test technology, and is widely used in the detection of male components in mixed components, individual identification and kinship identification. At present, there are more than 220 Y-STR loci co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143
Inventor 张更谦严江伟刘晋玎王荣帅
Owner 湖北崇新司法鉴定中心
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