Fluorescent carbon-dot nucleic acid probe based on fret mechanism and preparation method thereof
A nucleic acid probe and fluorescent carbon dot technology, applied in the fields of biomedicine and nanomaterials, can solve problems such as shortage, and achieve the effect of high agility and good repeatability
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[0020] The present invention will be further described in detail below in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the description.
[0021] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.
[0022] A fluorescent carbon-dot nucleic acid probe based on the FRET mechanism of this embodiment is a carbon-dot-acridine yellow complex. Its preparation method comprises the following steps:
[0023] 1) Prepare carbon dots;
[0024] 2) Using the obtained carbon dots and acriflavine to prepare a carbon dot-acridine yellow complex.
[0025] In one embodiment, the specific preparation method of the nucleic acid probe is:
[0026] 1. Preparation of carbon dots: Weigh 1.5g of citric acid, dissolve in 40mL of deionized water, stir evenly to dissolve completely, add 1mL of ethylenediamine with...
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