Fluorescent carbon-dot nucleic acid probe based on fret mechanism and preparation method thereof

A nucleic acid probe and fluorescent carbon dot technology, applied in the fields of biomedicine and nanomaterials, can solve problems such as shortage, and achieve the effect of high agility and good repeatability

Active Publication Date: 2021-06-22
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the use of carbon dots as fluorescent probes and applied to DNA detection has attracted more and more attention, but now there is a lack of nucleic acid probes based on carbon dots.

Method used

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  • Fluorescent carbon-dot nucleic acid probe based on fret mechanism and preparation method thereof
  • Fluorescent carbon-dot nucleic acid probe based on fret mechanism and preparation method thereof
  • Fluorescent carbon-dot nucleic acid probe based on fret mechanism and preparation method thereof

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Embodiment Construction

[0020] The present invention will be further described in detail below in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the description.

[0021] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0022] A fluorescent carbon-dot nucleic acid probe based on the FRET mechanism of this embodiment is a carbon-dot-acridine yellow complex. Its preparation method comprises the following steps:

[0023] 1) Prepare carbon dots;

[0024] 2) Using the obtained carbon dots and acriflavine to prepare a carbon dot-acridine yellow complex.

[0025] In one embodiment, the specific preparation method of the nucleic acid probe is:

[0026] 1. Preparation of carbon dots: Weigh 1.5g of citric acid, dissolve in 40mL of deionized water, stir evenly to dissolve completely, add 1mL of ethylenediamine with...

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Abstract

The invention discloses a fluorescent carbon-dot nucleic acid probe based on a FRET mechanism and a preparation method thereof. The nucleic acid probe is a carbon-dot-acridine yellow complex. The preparation method comprises the following steps: 1) preparing carbon dots; 2) preparing carbon dot-acridine yellow complexes by utilizing the obtained carbon dots and acriflavine. The present invention designs a carbon dot-acridine yellow composite system, which can effectively detect the concentration of target nucleic acid through FRET (fluorescence resonance energy transfer); the method is a ratio-based fluorescence detection method, which has high sensitivity and repeatability Good sex and other advantages.

Description

technical field [0001] The invention relates to the fields of biomedicine and nanomaterials, in particular to a fluorescent carbon-dot nucleic acid probe based on a FRET mechanism and a preparation method thereof. Background technique [0002] Nucleic acid is the genetic material of life, and its structural integrity determines whether cells can maintain normal physiological functions as well as cell division, proliferation, and differentiation. The integrity of the nucleic acid structure also determines the normal growth and development of living organisms, as well as the inheritance and variation in the reproduction process of living organisms. The diversity of DNA molecular structure has a great influence on its properties and functions. In the clinical chemical anti-cancer therapy, DNA is used as the target of chemical drugs, which is an effective means of cancer treatment, and DNA is also widely used in the diagnosis of diseases and the design of drugs. Therefore, sim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C09K11/65C12Q1/6818
CPCC09K11/06C09K11/65C09K2211/1029C12Q1/6818C12Q2563/107
Inventor 李力董文飞李林梅茜岳娟葛明锋常智敏邵昊华
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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