Method for quickly screening saccharification and/or ageing resisting substances

Abstract

The invention provides a method for quickly screening saccharification and / or ageing resisting substances. The method comprises the steps of feeding fruit flies of 1-3 days old with a cane sugar aqueous solution containing potential active substances to be tested, besides, using the fruit flies only fed with a cane sugar aqueous solution as a control group, then performing hungry treatment on thetwo groups of the fruit flies, finally, performing feeding with pyruvic aldehyde, and counting the death quantity of the fruit flies at intervals until the two groups of the fruit flies are dead completely; and when the mortality rate of the fruit flies in the control group is 40%-60%, performing statistics on the survival condition of the fruit flies in the experiment group, judging whether the potential active substances to be tested can prolong the survival time of the fruit flies or not, and if yes, determining that the substances are saccharification and / or ageing resisting substances. Within a 7-day experiment period, compared with a conventional cell model and other animal models, at an extremely low economy cost, whether one substance has activity of resisting saccharification andageing can be determined, and a prominent platform is provided for large-scale high-throughput screening of active substances for resisting diabetes and resisting ageing relevant diseases.

Description

technical field [0001] The invention belongs to the technical field of aging research, and in particular relates to a method for rapidly screening anti-glycation and / or anti-aging substances, which can discover anti-aging active substances in animal models within 7 days. Background technique [0002] With economic development, medical conditions have gradually improved, and the average life expectancy has also increased significantly. The average life expectancy of Chinese citizens has increased from 35 years old when New China was founded to 77 years old in 2018. At the same time, due to the gradual increase in the proportion of the elderly, my country has entered an aging society and is also the country with the largest elderly population in the world. Due to the increasing population of the elderly, the incidence of age-related diseases, such as diabetes, cancer, cardiovascular and cerebrovascular diseases, and neurodegenerative diseases, has increased year by year, resu...

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Problems solved by technology

Among them, yeast is a single-celled organism, and it is difficult to simulate the physiological response of multicellular organisms, while nematodes have less than 1,000 cells, which is too simple, genetically different from humans, lacks a complete nervous system, and is difficult to operate at the same time. Suitable for high-throughput screening; the lifespan of mice is 2-4 years, and even the shortest progeria model takes about 40 days, and the demand for potential active substances in mice is many times higher than that of Drosophila, so the mouse model High-throughput screening cannot be performed like the fruit fly model; the lifespan of monkeys is about 20 years old, the experimental period is too long, and the consumption of potential active substances is too large, so mice and monkeys are not suitable for testing potential potential in a very short period of time. Anti-aging drugs, while high-throughput screening is too expensive

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