A strain of Inonotus obliquus obtained by ultraviolet mutagenesis
A technology of Inonotus obliquus and strains is applied in the field of Inonotus obliquus strains obtained by ultraviolet mutagenesis, and can solve the problems of exceeding the standard of determination results, difficult to maintain a similar level of quality, complex raw material sources of bran and rice bran, etc.
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[0061] The Inonotus obliquus strain adopts the new Inonotus obliquus strain obtained by the present invention. The usage amount of rice bran is 2.0g / 100mL medium, the usage amount of bran is 3.0g / 100mL medium, add potassium dihydrogen phosphate 0.15g / 100mL, magnesium sulfate heptahydrate 0.075g / 100mL, pH is natural, add water to required sterilized at 121°C for 30min, used as a culture medium for fermentation, the shake flask loading was 40%, the inoculum was 10%, the cultivation temperature was 28°C, the rotation speed was 150r / min, and the cultivation time was 6d. The following steps (1) to (4) are implemented: (1) the mycelium containing a small amount of rice bran obtained by liquid culture is separated with a 20-mesh metal sieve, and washed twice with water; (2) the obtained The wet mycelium is extracted with multi-frequency ultrasonic-assisted warm water, the water temperature does not exceed 60 ° C, the clear liquid is filtered, and the qualified mycelium is obtained by...
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