A strain of Inonotus obliquus obtained by ultraviolet mutagenesis

A technology of Inonotus obliquus and strains is applied in the field of Inonotus obliquus strains obtained by ultraviolet mutagenesis, and can solve the problems of exceeding the standard of determination results, difficult to maintain a similar level of quality, complex raw material sources of bran and rice bran, etc.

Active Publication Date: 2022-06-21
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, we always hoped that the results of our research could deal with various bran and rice bran, so we intentionally relaxed the restrictions on the use of bran and rice bran raw materials, and did not formulate raw material standards. Some outliers in the research range, such as the problem of some heavy metal determination results exceeding the standard, are due to the complex sources of bran and rice bran raw materials, the quality is difficult to maintain a similar level, and unknown pollutants eventually enter the final product

Method used

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  • A strain of Inonotus obliquus obtained by ultraviolet mutagenesis
  • A strain of Inonotus obliquus obtained by ultraviolet mutagenesis
  • A strain of Inonotus obliquus obtained by ultraviolet mutagenesis

Examples

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Embodiment 1

[0061] The Inonotus obliquus strain adopts the new Inonotus obliquus strain obtained by the present invention. The usage amount of rice bran is 2.0g / 100mL medium, the usage amount of bran is 3.0g / 100mL medium, add potassium dihydrogen phosphate 0.15g / 100mL, magnesium sulfate heptahydrate 0.075g / 100mL, pH is natural, add water to required sterilized at 121°C for 30min, used as a culture medium for fermentation, the shake flask loading was 40%, the inoculum was 10%, the cultivation temperature was 28°C, the rotation speed was 150r / min, and the cultivation time was 6d. The following steps (1) to (4) are implemented: (1) the mycelium containing a small amount of rice bran obtained by liquid culture is separated with a 20-mesh metal sieve, and washed twice with water; (2) the obtained The wet mycelium is extracted with multi-frequency ultrasonic-assisted warm water, the water temperature does not exceed 60 ° C, the clear liquid is filtered, and the qualified mycelium is obtained by...

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Abstract

The invention discloses an Inonotus obliquus strain obtained by ultraviolet mutagenesis, which relates to the application field of food microorganism technology. The new strain of Inonotus obliquus has a preservation number of CCTCC NO: M 2018906, and the recommended Latin name is Inonotus obliquus Bacteria JSU LIUK18, the Latin name is Inonotus obliquus JSU LIUK18. The new strain of Inonotus obliquus is used for liquid fermentation in a culture medium mainly composed of whole bran and rice bran which has been tested to meet the pollutant limit use standard, to produce the fermented product of Inonotus obliquus mycelium raw material and fermentation liquid Finally, through multi-frequency ultrasonic-assisted extraction, freeze concentration, citric acid extraction to replace heavy metal ions, alcohol precipitation and alcohol washing, and freeze-drying to obtain qualified Inonotus obliquus polysaccharide products.

Description

technical field [0001] The invention relates to the technical application field of food microorganisms, in particular to a new strain of Inonotus obliquus obtained by ultraviolet mutagenesis, which is used for the cultivation of bran and rice bran whole materials as main components which have been tested in liquid fermentation and reach the limited use standard of pollutants. After producing the raw material of Inonotus obliquus mycelium and the fermented product of the fermentation broth, the qualified Chaga is obtained through multi-frequency ultrasonic-assisted extraction, freeze concentration, citric acid leaching to replace heavy metal ions, alcohol precipitation and alcohol washing, and freeze-drying. Polysaccharide products of pore bacteria. Background technique [0002] Many medicinal and edible fungi or medicinal fungi have a long history of use and a wide range of people in China. Modern science has revealed that the mycelium, fruiting bodies or spores of rare fun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01H15/00C12N13/00C12R1/645
CPCA01H15/00C12N13/00C12R2001/645C12N1/145
Inventor 刘伟民卡万姆·阿塔富·阿木福艾志录余昭玮汪涛杨卫卫沈国栋马海乐
Owner JIANGSU UNIV
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