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Nucleic acid detection test strip for distinguishing Brucella melitensis vaccine strain S19 from natural infection strain

A technology of Brucella, detection test paper, applied in the direction of determination/inspection of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc. Infectious strain detection and other issues to achieve high specificity and sensitivity

Pending Publication Date: 2020-01-31
ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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Problems solved by technology

[0006] In order to solve the problem that existing pathogen detection methods cannot simultaneously detect Brucella vaccine strain S19 and natural infection strains, the invention provides a nucleic acid detection test strip for distinguishing Brucella vaccine strain S19 and natural infection strains

Method used

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  • Nucleic acid detection test strip for distinguishing Brucella melitensis vaccine strain S19 from natural infection strain
  • Nucleic acid detection test strip for distinguishing Brucella melitensis vaccine strain S19 from natural infection strain
  • Nucleic acid detection test strip for distinguishing Brucella melitensis vaccine strain S19 from natural infection strain

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[0021] A nucleic acid detection test strip for distinguishing Brucella vaccine strain S19 from natural infection strains of the present invention, its main feature is to use biotin and / or fluorescent groups to label primers and / or probes, using two A labeled chromatographic test strip for rapid detection of nucleic acid amplification products. Wherein, the nucleic acid amplification product is obtained by amplifying the specific fragments of the Brucella vaccine strain S19 and the natural infection strain.

[0022] A kind of nucleic acid detection test strip that distinguishes Brucella vaccine strain S19 and natural infection strain of the present invention mainly comprises:

[0023] Brucella forward primer sequence F1: catccttacgcgcaacgatatggatcgtttcc.

[0024] Brucella reverse primer sequence R1: biotin-tttccggcgcaggcgaaaagggcgtgccgggtctc.

[0025] Brucella probe sequence Probe1: FAM (or FITC)-accttgcccttgccatcataaaggccggtgc(dSpacer)gttataggcccaataggc-P.

[0026] Brucella...

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Abstract

The invention relates to a nucleic acid detection test strip for distinguishing a Brucella melitensis vaccine strain S19 from a natural infection strain, relates to the field of Brucellosis detection,and solves the problems that a conventional pathogeny detection method cannot detect the Brucella melitensis vaccine strain S19 and the natural infection strain at the same time. The detection test strip comprises a Brucella melitensis forward primer of which the sequence is shown as SEQID NO:1, a Brucella melitensis reverse primer of which the sequence is shown as SEQID NO:2, and a Brucella melitensis probe of which the sequence is shown as SEQID NO:3. The sequence of the forward primer of the Brucella melitensis vaccine strain S19 is shown as SEQID NO:4, the sequence of the reverse primer of the Brucella melitensis vaccine strain S19 is shown as SEQID NO:5, and the sequence of the probe of the Brucella melitensis vaccine strain is shown as SEQID NO:6. The test strip can specially detectthe disferences between Brucella melitensis natural infection and artificial immunity, and has high specificity and sensibility.

Description

technical field [0001] The invention relates to the technical field of brucellosis detection, in particular to a dual detection card for Brucella vaccine strain S19 and natural infection strain and a preparation and use method thereof. [0002] technical background [0003] Brucella is an important zoonotic pathogen, which plays an important role in animal husbandry. In recent years, the outbreak of brucellosis in my country has become a very strong threat. The state has issued the "National Medium and Long-Term Plan for Brucellosis (2016-2020)", which stipulates the quarantine and related prevention and control plans for Brucella , which shows the important role of this disease in the prevention and control of epidemics in my country. [0004] At present, the brucellosis of economic animals such as cattle and sheep is mainly prevented and cured by methods such as vaccine immunization and quarantine clearance. Currently, the S19 strain (Brucella bovis) and S2 strain (Brucelo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6844C12Q1/04C12R1/01
CPCC12Q1/689C12Q1/6844C12Q2565/625C12Q2563/107
Inventor 郎需龙卜昭阳祝令伟
Owner ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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