Mutation type PSII D1 or psbA D1 protein, and coding nucleic acid and application thereof

A mutant, protein amino acid technology, applied in the field of agricultural genetic engineering

Inactive Publication Date: 2020-02-07
QINGDAO KINGAGROOT CHEM COMPOUNDS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, rice cultivation faces serious challenges from weedy rice
Although the current rice varieties resistant to imidazolinone (acetolactate synthase, ALS) and acetyl-CoA carboxylase (ACCase) inhibitors can control weedy rice, after 3-4 years of use, weedy rice will Resistant to herbicides like rice

Method used

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  • Mutation type PSII D1 or psbA D1 protein, and coding nucleic acid and application thereof
  • Mutation type PSII D1 or psbA D1 protein, and coding nucleic acid and application thereof
  • Mutation type PSII D1 or psbA D1 protein, and coding nucleic acid and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] In the present invention, a large number of seeds are obtained by planting the mutated rice seeds, and then a large number of second-generation rice seedlings are obtained by sowing, spraying PSII herbicides, sequencing and analyzing the surviving seedlings, the specific steps are as follows:

[0061]Purchase 100 kilograms of Huaidao No. 5 and 100 kilograms of Huanghuazhan seeds from the market, soak them in water for 22 hours in an artificial climate chamber at 28°C, remove and dry the floating water, and then use 0.7% ethyl methanesulfonate (Ethyl methanesulfonate, EMS) Soak for 12 hours. Stir once an hour during this period, so that the ethyl methanesulfonate can be fully and evenly absorbed by the seeds. In addition, part of the seeds were taken, soaked in water again for 12 hours, and treated without adding ethyl methanesulfonate as a control. After 12 hours, pick up the seeds, discard the ethyl methanesulfonate aqueous solution, and rinse repeatedly with a large ...

Embodiment 2

[0066] The resistant rice seedlings of the present invention are cultivated and seeded, and crossed with the wild type. It is found that the mutant type is used as the female parent, and the wild type pollen is used to pollinate the mutant type female parent, and the new seeds obtained all inherit the PSII inhibitor. Drug resistance; conversely, the wild type is used as the female parent, and the pollen of the mutant plant is used to pollinate the wild type, and all the resulting plants are killed. The specific method is as follows:

[0067] According to the sequencing results of rice seedlings, two sets of hybridization experiments were carried out directly using contemporary surviving plants and wild-type plants. One group is Huaidao 5 containing the S264G mutation from atrazine screening and the wild type Huaidao 5, and the other group is Huang Huazhan and the wild type Huang Huazhan containing the V219I mutation selected from Diuron. Fifty healthy rice plants with similar...

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Abstract

The invention belongs to the field of agriculture genetic engineering, and particularly relates to mutation type PSII D1 or psbA D1 protein, and coding nucleic acid and application thereof. Compared with the amino acid sequence of wild type rice PSII D1 or psbA D1 protein, the amino acid sequence of the mutation type PSII D1 or psbA D1 protein has the characteristics that one or more mutants selected from the following 219I, 249A, 251V, 255Y, 256A, 264G, 264A, 264T, 266T and 275F exist at one or more positions corresponding to the 219th, the 249th, the 251st, the 255th, the 256th, the 264th, the 266th and the 275th sites in the amino acid sequence of the wild type rice PSII D1 or psbA D1 protein as shown corresponding to SEQ ID. 04. Through the mutation sites, the capacity for resisting PSII restraining herbicides is given to rice, so that the problem that weedy rice and other crops are seriously subjected to phytotoxicity of herbicides can be solved.

Description

technical field [0001] The invention belongs to the field of agricultural genetic engineering, and specifically relates to mutant PSII D1 or psbA D1 protein, its encoding nucleic acid and application. Background technique [0002] Rice is one of the most important food crops in the world, feeding nearly half of the world's population, especially in Asia. Rice is the absolute food of life for people in Asia. However, rice cultivation faces serious challenges from weedy rice. Although the current rice varieties resistant to imidazolinone (acetolactate synthase, ALS) and acetyl-CoA carboxylase (ACCase) inhibitors can control weedy rice, after 3-4 years of use, weedy rice will Herbicide resistant like rice. The herbicide resistance traits of rice will follow the hybridization between rice and weedy rice, that is, the pollen of resistant rice carries these herbicide resistance genes to pollinate weedy rice, resulting in the resistance of weedy rice. With the further developmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K19/00C12N15/29C12N15/62C12N15/82A01H5/00A01H6/46C12Q1/6895
CPCC07K14/415C07K2319/08C07K2319/21C12N15/8274C12Q1/6895C12Q2600/158A01H1/06C12N5/14C12N15/82C12Q1/68
Inventor 连磊李华荣莫苏东彭学岗
Owner QINGDAO KINGAGROOT CHEM COMPOUNDS CO LTD
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