Method for inducing formation of oyster mushroom primordia

A technology of oyster mushroom and plate culture medium, applied in botany equipment and methods, mushroom cultivation, horticulture, etc., can solve the problems of long fruiting body formation period of oyster mushroom and high cultivation cost of oyster mushroom, shorten the fruiting time, shorten Formation time, effect of increasing fruiting yield

Inactive Publication Date: 2020-02-11
INST OF PLANT NUTITUION & RESOURCE ENVIRONMENT HENAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The three induction stimulation methods need at least 5 days to make the mycelia of Pleurotus ostreatus form the primordium of Pleurotus ostreatus, and there is still a technical defect that the fruiting body of Pleurotus ostreatus is relatively long, which leads to higher cultivation costs of Pleurotus ostreatus

Method used

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  • Method for inducing formation of oyster mushroom primordia
  • Method for inducing formation of oyster mushroom primordia

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1 Adopt the induction method of the present invention to induce the Pleurotus ostreatus hyphae (i.e. P99 mycelium) in the traditional cultivation bag to form the Pleurotus ostreatus primordium, the specific method is:

[0025] In the first step, P99 P99 that was stored in low temperature or frozen in liquid nitrogen was taken out, inoculated on PDA plate culture medium (20 dishes in total) and activated at 25°C for 8-10 days to obtain activated P99 mycelia;

[0026] In the second step, the P99 mycelium with growth potential of ++ or +++ in the first step is screened out, and the screened P99 mycelium is punched with a puncher (inner diameter of 5 mm), and the punched mycelium is Blocks were inoculated into PDA medium (one mycelia block was inoculated in each petri dish, a total of 50 dishes were inoculated) and activated at 25°C for 8-10 days to obtain secondary activated P99 mycelia;

[0027] In the third step, the P99 mycelium obtained by secondary activati...

Embodiment 2

[0034] Embodiment 2 Adopt the induction method of the present invention to induce the Pleurotus ostreatus hyphae (i.e. P99 mycelium) in the cultivation bottle to form the Pleurotus ostreatus primordium, the specific method is:

[0035] In the first step, P99 P99 that was stored in low temperature or frozen in liquid nitrogen was taken out, inoculated on PDA plate culture medium (20 dishes in total) and activated at 25°C for 8-10 days to obtain activated P99 mycelia;

[0036] In the second step, the P99 mycelium with growth potential of ++ or +++ in the first step is screened out, and the screened P99 mycelium is punched with a puncher (inner diameter of 5 mm), and the punched mycelium is Blocks were inoculated into PDA medium (one mycelia block was inoculated in each petri dish, a total of 50 dishes were inoculated) and activated at 25°C for 8-10 days to obtain secondary activated P99 mycelia;

[0037] In the third step, the P99 mycelium activated twice in the second step is p...

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Abstract

The invention discloses a method for inducing formation of oyster mushroom primordia. The method includes the following steps: step 1, inoculating a PDA plate culture medium with an oyster mushroom strain to activate and cultivate; step 2, punching oyster mushroom hyphae obtained through activation in the step 1 through a punching device to obtain hypha blocks, and inoculating the PDA plate culture medium with the hypha blocks to activate and cultivate; step 3, respectively inoculating cultivation bags or bottles filled with cultivation materials with oyster mushroom hyphae obtained through activation in the step 2, and performing cultivation until the bags or bottles are full of the hyphae; step 4, after the bags or bottles are full of the hyphae, spraying auxin into each cultivation bagor bottle, and forming openings to induce the oyster mushroom hyphae to form the oyster mushroom primordia. The method has the advantages that the auxin used for stimulating the hyphae to form the oyster mushroom primordia is cheap and easy to obtain, the spraying concentration of the auxin can be as low as 10<-8>M, the consumption is low, the formation time of the oyster mushroom primordia can beshortened, the fruiting time of oyster mushrooms can be further shortened, the fruiting yield of the oyster mushrooms can be effectively increased, and economic benefits are high.

Description

technical field [0001] The invention relates to the cultivation technology of Pleurotus ostreatus, in particular to a method for inducing the formation of Pleurotus ostreatus primordia. Background technique [0002] oyster mushroom ( Pleurotus otreatus ) is scientifically known as Pian Er, which is delicious, nutritious, and has high edible value. It has the reputation of "meat among vegetarians" and is deeply loved by consumers. In recent years, consumers' demand for oyster mushrooms is increasing day by day. In order to meet the market demand, industrialized Pleurotus ostreatus bottle cultivation technology has emerged in various parts of Henan Province, that is, using cultivation bottles for cultivation. It is widely accepted because of its advantages such as high yield, high quality, reduced drug use, and reduced cost. welcome. However, regardless of the traditional cultivation mode or the industrial bottle cultivation or bag cultivation mode, how to shorten the growt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/40
CPCA01G18/00A01G18/40
Inventor 崔筱孔维丽孔维威张玉亭康源春袁瑞奇刘芹胡素娟段亚魁宋志波韩玉娥
Owner INST OF PLANT NUTITUION & RESOURCE ENVIRONMENT HENAN ACADEMY OF AGRI SCI
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