Multi-channel droplet quantitative measuring device and measuring method based on pressure driving

A multi-channel, droplet technology, applied in the microfluidic field of analytical chemistry, can solve the problem that it cannot be used to quantitatively measure liquids of unknown viscosity, and achieve the effects of easy large-scale parallelization, convenient operation and simple structure

Active Publication Date: 2020-02-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] By utilizing the characteristics of multi-phase liquid droplets, the present invention solves the defect that the traditional pressure-driven liquid quantitative measurement method cannot be used to quantitatively measure unknown viscosity liquids

Method used

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  • Multi-channel droplet quantitative measuring device and measuring method based on pressure driving
  • Multi-channel droplet quantitative measuring device and measuring method based on pressure driving
  • Multi-channel droplet quantitative measuring device and measuring method based on pressure driving

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] This example applies the present invention to the screening of protein crystallization conditions. Such as figure 1 and figure 2 As shown, a pressure-driven multi-channel liquid droplet quantitative measuring device includes:

[0083] Pressure source and pressure control system 1, which can provide positive and negative pressure sources and control the magnitude and application time of pressure;

[0084] The working medium reservoir 3 is used to hold the working medium 2 with known viscosity-temperature relationship;

[0085] Temperature sensor 8, capable of measuring the working environment temperature;

[0086] The fluid damper 4, its liquid inlet end is connected with the working medium liquid storage pool 3 and communicated with its medium cavity;

[0087] The sampling probe 7 is hermetically connected with the liquid outlet end of the fluid damper 4 through the connector 23;

[0088] The fixture 6 is used for positioning and fixing the array formed by the spo...

Embodiment 2

[0102] In this embodiment, a droplet array composed of different drug solutions is firstly generated, and a quantitative dilution operation is performed on the droplets therein.

[0103] Such as image 3 As shown, in this embodiment, the multi-channel droplet quantitative measuring device is similar to that of Embodiment 1, but the number of channels is 6. The multi-channel droplet quantitative measuring device is also installed on the automatic translation platform, and the automatic translation platform is controlled by a computer program.

[0104] In this embodiment, the porous plate 9 is a 24-well plate, the sample solution 10 is an aqueous solution of 24 different drugs, the chip 11 is a glass chip with micropits, and the working medium 12 is mineral oil. Another liquid storage tank 19 contains pure water 20 for dilution.

[0105] In this embodiment, the multi-channel droplet quantitative measuring device first quantitatively draws 10 nL of the drug solution from the po...

Embodiment 3

[0108] This embodiment is a highly integrated and modified version of the present invention.

[0109] Such as Figure 4 As shown, in this embodiment, the pressure source and pressure control system 1 is composed of an air compressor, a vacuum pump and a servo air pressure controller. The compressed air and vacuum required by the servo air pressure controller are provided by the air compressor and the vacuum pump respectively. By adopting the servo air pressure controller, the pressure source and the pressure control system 1 can generate continuously adjustable air pressure of -0.1MPa-0.6MPa. The pressure source and the pressure control system 1 are finally controlled by a computer program.

[0110] In this embodiment, the working medium reservoir 3 , the fluid damper 4 , and the sampling probe 7 are integrated into a microfluidic chip 21 . The working medium liquid reservoir 3 is connected with the pressure source and the pressure control system through the air pressure in...

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Abstract

The invention discloses a multi-channel droplet quantitative measuring device based on pressure driving. The multi-channel droplet quantitative measuring device comprises a sample application probe for performing sample application operation on a sample solution, a working medium liquid storage tank for containing a working medium, a pressure control system for controlling the pressure in the working medium liquid storage tank, and a fluid damper provided with a micro-channel structure and having a damping effect on fluid in the micro-channel structure, wherein the liquid inlet end of the fluid damper communicates with the medium liquid storage tank, and the liquid outlet end of the fluid damper communicates with the liquid inlet end of the sample application probe in a sealed mode. The liquid droplet quantitative measuring device has the liquid droplet quantitative measuring capacity from picoliter level to microliter level and the liquid droplet quantitative measuring precision as low as picoliter level. According to the multi-channel droplet quantitative measuring device, various solutions with different viscosities can be measured in parallel, and the capability is an essentialcapability for large-scale high-throughput screening applications such as drug screening, protein crystallization condition screening and the like which need to treat a large amount of solutions withdifferent properties. The device is easy to construct, convenient to operate and good in reliability.

Description

technical field [0001] The invention relates to the microfluidic field of analytical chemistry, in particular to a pressure-driven multi-channel droplet quantitative measuring device and measuring method. Background technique [0002] A droplet is a discontinuous fluid in which one phase of liquid is separated from another immiscible phase or multiphase liquid. Among them, the discontinuous phase that constitutes the droplets is called the dispersed phase, and the liquid that separates the droplets is called the continuous phase. In the field of microfluidics, the most common droplet system uses aqueous solution as the dispersed phase, and mineral oil or fluorine oil as the continuous phase, where the droplet volume is usually from picoliters to nanoliters. [0003] In the previous research, the inventor's research group developed a liquid droplet quantitative measurement and operation method. Through the cooperation of precision syringe pump, micro sampler and sharpened ca...

Claims

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Application Information

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IPC IPC(8): B01L3/02
CPCB01L3/0293
Inventor 王慧峰方群
Owner ZHEJIANG UNIV
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