Hyaluronic acid gel for hyaluronic acid injection and use method of hyaluronic acid gel

A technology of hyaluronic acid and cross-linked hyaluronic acid, applied in the field of biomedicine, can solve the problems of short maintenance time of moisturizing effect, low toxicity of impurities, unsuitability, etc., achieve moisturizing effect, improve skin texture, and low strength Effect

Pending Publication Date: 2020-02-21
BIOREGEN BIOMEDICAL (CHANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem in the prior art that the hyaluronic acid injected by water light is degraded and absorbed in the body, the maintenance time of the moisturizing effect is short, and at the same time, in order to solve the problem that the cross-linked hyaluronic acid gel in the prior art has a long degradation and absorption time, but it is not suitable for For the problem of water-light injection, this application provides a disulfide cross-linked hyaluronic acid gel for water-light injection, which has a longer degradation and absorption time and longer moisturizing maintenance than the existing hyaluronic acid. time, impurities are less toxic, and are suitable for water-light injections, and will not produce the bulging phenomenon that is easy to occur in current cross-linked hyaluronic acid gel products, so water-light injections can be used to improve skin quality more effectively

Method used

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  • Hyaluronic acid gel for hyaluronic acid injection and use method of hyaluronic acid gel
  • Hyaluronic acid gel for hyaluronic acid injection and use method of hyaluronic acid gel
  • Hyaluronic acid gel for hyaluronic acid injection and use method of hyaluronic acid gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Preparation of disulfide cross-linked hyaluronic acid gel

[0029] Hyaluronic acid thiolated derivatives were prepared from sodium hyaluronate with a molecular weight of 180KDa by the method reported by Shu et al. (Shu et al., Biomacromolecules 2002, 3:1304-1311), and the thiol content was 24 μmol / g and 38 μmol, respectively. / g and 57 μmol / g.

[0030] Dissolving the above-mentioned hyaluronic acid thiolated derivative to obtain 10ml aqueous solution, the contents are respectively 3, 4, 5.5, 6.5, 7, 8 and 10mg / mL; adjust the pH value of the solution to 7.4, transfer into 25ml sterile glass after sterilization filtration Container; airtight and stored at room temperature for 4 weeks, the solution loses fluidity and forms a disulfide cross-linked hydrogel.

Embodiment 2

[0031] Example 2: Evaluation of Injectable Properties of Disulfide Cross-Linked Hyaluronic Acid Gels

[0032] Adopt Derma (DeMarsha) 2nd generation water-light injection instrument, test the injectability of the gel prepared in Example 1 through conventional water-light injection needles (nine needles), and the test results are as follows:

[0033]

[0034] When the content of cross-linked hyaluronic acid is 3mg / mL and 4mg / mL, it usually has good injectability; while the content is ≥ 5.5mg / mL, it is difficult to inject through the water-light injector.

Embodiment 3

[0035] Example 3: Evaluation of injectable properties of disulfide-crosslinked hyaluronic acid gels after dilution

[0036] The gel prepared in Example 1 (the content of cross-linked hyaluronic acid was: 5.5 mg / mL, 6.5 mg / mL, 7 mg / mL, 8 mg / mL and 10 mg / mL; the thiol content of the thiolated derivative was 38 μmol / g) Physically mixed and diluted with normal saline at volume ratios of 1:1, 1:1.5 and 1:2. The dilution process was carried out in syringes connected with two-way joints, one of the syringes was filled with the gel prepared in Example 1 (1mL), and the other was filled with an appropriate volume of physiological saline (1mL, 1.5mL, 3mL), Push back and forth 60 to 80 times for physical dilution and mixing.

[0037] Adopt Derma (DeMartha) 2nd generation hydro-optical injection instrument to test the injectability of the diluted gel through conventional hydro-optical injection needles (nine needles). The test results are as follows:

[0038]

[0039] When diluted ...

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Abstract

The invention discloses a hyaluronic acid gel for hyaluronic acid injection. The hyaluronic acid gel is a disulfide crosslinked hyaluronic acid, and the content of hyaluronic acid in the gel is not more than 10 mg/mL. The crosslinked hyaluronic acid gel provided by the invention has unique advantages when used for hyaluronic acid injection, is easily injected through a very fine needle head of a hyaluronic acid injection needle, and can realize good and uniform distribution in a subcutaneous layer without bulging; and the hyaluronic acid gel has a moisturizing effect longer than that of uncrosslinked hyaluronic acid, and can effectively improve skin texture.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to a hyaluronic acid gel for hydro-optic injection, and a method for using the hyaluronic acid gel for hydro-optic injection. Background technique [0002] In recent years, the medical beauty industry at home and abroad has developed rapidly, and various emerging beauty technologies have been developed. Water light injection is one of the most extensive medical cosmetology projects at home and abroad. It can effectively solve the problem that skin care products used on the body surface are not easily absorbed through the epidermal barrier. senescence. Water-light injection originated from Mesotherapy proposed by French doctor Michal Pistor in 1952. Its concept is to inject a small amount of therapeutic substances in the local superficial surface; after decades of hard work, the current water-light injection technology was developed. Shuiguang injection uses negative pressure tec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/04A61K8/73A61Q19/00
CPCA61K8/042A61K8/735A61K2800/91A61Q19/00
Inventor 王云云王坤张红晨胡慕兰王昕宇宋文俊舒晓正
Owner BIOREGEN BIOMEDICAL (CHANGZHOU) CO LTD
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