Method for preventing degeneration of longya lilium
A technology of Lilium longya and culture medium formula, which is applied in the field of Lilium longya breeding, can solve the problems of Lilium longya degeneration, Lily longya species degeneration, and limited detoxification effect of stem tips, etc., to achieve increased yield, high reproduction coefficient, single weight effect
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[0034] The present embodiment provides a kind of method that prevents lily of dragon tooth from degenerating, specifically comprises the following steps:
[0035] S1. Seed selection: look for the native species of Lilium longya in the wild, take its fruit as an explant, and carry out sterilization treatment: rinse the fruit with tap water, dry it, then soak it with 75% alcohol for 30 seconds, and then put it in dry on an alcohol lamp;
[0036] S2. Cultivation: Cut the treated fruit in step S1, peel off the embryo and inoculate it on the medium for primary culture, subculture and rooting culture, wherein the medium formula for the primary culture is MS+0.3mg / L6 -BA+sucrose 3%, the medium formula of the 2nd to 5th generations is MS+0.2mg / L 6-BA+3% sucrose, the medium formula of the 5th to 10th generations is MS+0.02mg / L 6-BA+3% sucrose;
[0037] S3. Seedling hardening and transplanting: the hardening process is carried out in a greenhouse at an altitude of 1000m. The hardening ...
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