Method for determining asarinin in blood-nourishing and brain-refreshing water extract

Abstract

The invention relates to a method for determining asarinin in blood-nourishing and brain-refreshing water extract. The method comprises the following steps: 1) preparation of a reference substance solution: weighing a proper amount of asarinin reference substance, and adding methanol to dissolve to obtain the asarinin-containing reference substance solution; 2) preparation of a test solution: taking the blood nourishing and brain refreshing water extract, adding 40-60% (v / v) of methanol, ultrasonically dissolving, passing through a solid phase extraction column, washing with 50-60% of methanol, discarding, eluting with a pure methanol solution, collecting eluent, and adding methanol to a scale to obtain the test solution; and 3) detection: respectively taking the reference solution and thetest solution, injecting the reference solution and the test solution into an ultra-high performance liquid chromatograph, recording a chromatogram, calculating the asarinin content of the test solution by using an external standard method according to the peak area, and converting to obtain the asarinin content in the blood nourishing and brain refreshing aqueous extract.

Description

technical field [0001] The invention relates to a method for detection and analysis of medicinal components, in particular to a method for determination of asarin in brain water extract of nourishing serum. Background technique [0002] Yangxueqingnao Granules and Yangxuexuenao Pills are Chinese patent medicines developed by Tasly Group, which are recorded in pages 1281-1282 and 1280-1281 of the 2015 edition of the Chinese Pharmacopoeia, respectively. During the preparation process of Yangxuexuenao Granules and Yangxuexuenao Pills, it is necessary to extract Chinese medicinal materials such as Rehmannia glutinosa, Uncaria, Caulis Spatholobus, Prunella vulgaris, mother of pearl, and Asarum, and prepare them into Chinese medicine extract extracts. The extract of traditional Chinese medicine extract is one of the various preparation intermediates of Yangxuexuenao Granules and Yangxuexuenao Pills. The medicinal material is decocted twice with water, filtered, the filtrate is co...

AI Technical Summary

Problems solved by technology

[0008] However, in the detection method disclosed in the prior art, the target detection sample has a single medicinal taste, poor extraction and purification effect, low chromatographic separation, unsatisfactory precision and accuracy, and cannot To achieve the determination of asarin in the water extract of Yangxue Xuenao, this application adopts a new technical solution, adopts ultra-high performance liquid chromatography, and uses reversed-phase silica gel solid-phase extraction as a pretreatment method. Only methanol is used as a reagent, which minimizes the use of organic reagents, simpler operation, and better uniformity of commercial products

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