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Brown planthopper NlAtg1 gene and application thereof and encoded protein and application thereof

A technology of gene coding and brown planthopper, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Inactive Publication Date: 2020-04-17
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interference of the ATG1 gene can affect autophagy in other species, but mostly at the cellular level

Method used

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  • Brown planthopper NlAtg1 gene and application thereof and encoded protein and application thereof
  • Brown planthopper NlAtg1 gene and application thereof and encoded protein and application thereof
  • Brown planthopper NlAtg1 gene and application thereof and encoded protein and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1 Materials and methods

[0027] 1.1 Tested brown planthopper

[0028] The tested N. lugens population is the Tn population raised on the susceptible rice variety TN1. The laboratory has continuously raised more than 60 generations on TN1. The breeding temperature is 26±2°C, the relative humidity is 80%±5%, and the photoperiod is 12L: 12D.

[0029] main reagent

[0030] TaKaRa MiniBEST Universal RNA Extraction Kit, TaKaRa MiniBEST Agarose GelDNA Extraction Kit, PrimeScript RT reagent Kit With gDNA Eraser, DNA 2000Marker, Premix Taq ™ (TaKaRa Taq ™ Version 2.0 plus dye), SYBR Premix Ex Taq were purchased from Dalian TaKaRa Company, SMARTer® RACE 5' / 3' Kit User Manual was purchased from American Clontech Company, MEGAscript® T7 High Yield Transcription Kit was purchased from American Ambion Company, sequencing and The primers were synthesized by Shanghai Sunny Biotechnology Co., Ltd.

[0031] brown planthopper NlAtg1 Cloning of full-length gene cDNA

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Abstract

The invention discloses a brown planthopper NlAtg1 gene and application thereof and an encoded protein and application thereof. The nucleotide sequence of the NlAtg1 gene is shown as SEQ ID NO: 1, themitochondrial structure and function of the brown planthopper can be influenced when the function of the gene is inhibited, so that the survival rate of the brown planthopper is reduced. The amino acid sequence of the protein encoded by the NlAtg1 gene is shown as SEQ ID NO: 2. According to the application of the NlAtg1 gene or protein, the NlAtg1 gene or protein can be used for developing a novel RNA insecticide in brown planthopper prevention and treatment or researching and developing a pesticide taking the NlAtg1 protein as a target. The RNA interference is successfully carried out on thegene; the result shows that the survival rate of pests is reduced; since RNA interference fragments can be specifically designed for species, the killing of non-target organisms is expected to be reduced while the pests are inhibited. The formed insecticide is an environment-friendly insecticide.

Description

technical field [0001] The invention relates to a brown planthopper NlAtg1 gene, encoded protein and application thereof. Background technique [0002] Brown planthopper ( Nilaparvata lugens Stål) is an important rice pest. At present, the emergency control of brown planthopper is mainly based on chemical control. While chemical pesticides kill pests, they will also endanger non-target organisms including natural enemies, which will inevitably have a negative impact on the farmland ecosystem, resulting in the failure of the ecological control function to function as it should. Therefore, it is of great practical significance to screen and discover new targets for the control of N. lugens. Autophagy is a highly regulated self-degradation process that maintains the physiological homeostasis and survival of cells by removing excess proteins, organelles, and invading pathogens. Autophagy is regulated by a series of ATG (autophagy related gene) core genes and at least four i...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/435A01N57/16A01P7/04
CPCA01N57/16C07K14/43563A01N63/60A01N25/006
Inventor 郝培应俞晓平俞飞飞马正张蓬军申屠旭萍许益鹏王正亮刘光富杨倩倩庞琨
Owner CHINA JILIANG UNIV
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