Method for regulating and controlling aggregation state of human islet protein and medicine for treating and preventing type 2 diabetes mellitus
A technology of protein aggregation and islets, which is applied in drug combination, metabolic diseases, active ingredients of anhydride/acid/halide, etc., can solve problems such as out-of-control blood sugar regulation, achieve the effect of improving survival rate, reducing survival time, and improving survival rate
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[0030] This embodiment provides a method for regulating the aggregation state of human insulin, including the following steps:
[0031] 1. Mix the fast green powder and deionized water ultrasonically and shake for 1 minute under 200w conditions to form a 1 mM fast green solution;
[0032] 2. Mix hIAPP with phosphate buffered saline solution to form a 500μM protein solution;
[0033] 3. Add deionized water to the protein solution and dilute with shaking to reduce the concentration of the protein solution to 50μM;
[0034] 4. Divide the 1mM fast green solution into three parts, and then dilute them into 8μM, 80μM and 800μM fast green solutions;
[0035] 5. Take out 40 μL from the protein solution, and then add 40 μL of deionized water to form a blank group with a hIAPP concentration of 25 μM;
[0036] 6. Take out 40μL from the protein solution, and then add 40μL of 8μM fast green solution to form sample 1 with hIAPP concentration of 25μM and fast green concentration of 4μM;
[0037] 7. Take...
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