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Efficient preparation method of Sparassis crispa polysaccharides

A technology of bacterial polysaccharides and hydrangea, which is applied in the field of hydrangea extraction, can solve the problems that there are no related literature disclosures or reports, and achieve the effects of increasing permeability, facilitating release, and improving purity

Inactive Publication Date: 2020-04-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] At present, there is no relevant publication or report on the research on the extraction of hydrangea polysaccharides by high-voltage pulsed electric field.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A high-efficiency preparation method of hydrangea polysaccharides, comprising the steps of:

[0044] (1) Hydrangea pretreatment: Hydrangea fruiting bodies are vacuum-dried at 60°C to constant weight, crushed, and passed through a 60-mesh sieve for later use;

[0045] (2) Get the pretreated Hydrangea powder, add 10 times the weight of 5vol% dimethyl sulfoxide aqueous solution, and extract in a bipolar square wave high-voltage pulsed electric field at a temperature of 40 ° C, wherein the pulsed electric field intensity is 20Kv / cm, the frequency is 400Hz, the processing time is 0.5h, and the extract is collected;

[0046] (3) Microwave extraction was performed on the extract, the microwave power was 300W, the extraction temperature was 100°C, and the extraction time was 2min;

[0047](4) Centrifuge the extract after microwave extraction, concentrate the supernatant to a certain volume under reduced pressure, and use absolute ethanol alcohol equivalent to three times the ...

Embodiment 2

[0054] Referring to Example 1, a high-efficiency preparation method of a hydrangea polysaccharide of the present embodiment comprises the following steps:

[0055] (1) Hydrangea pretreatment: Hydrangea fruiting bodies are vacuum-dried at 60°C to constant weight, crushed, and passed through a 60-mesh sieve for later use;

[0056] (2) Get the hydrangea powder after pretreatment, add 20 times the weight of 8vol% dimethyl sulfoxide aqueous solution, and place it in a bipolar square wave high-voltage pulsed electric field at a temperature of 40°C to extract, wherein the pulsed electric field strength is 30Kv / cm, the frequency is 600Hz, the treatment time is 1h, and the extract is collected;

[0057] (3) The extract was subjected to microwave extraction, the microwave power was 400W, the extraction temperature was 100°C, and the extraction time was 2min;

[0058] (4) Centrifuge the extract after microwave extraction, concentrate the supernatant to a certain volume under reduced pr...

Embodiment 3

[0060] Referring to Example 1, a high-efficiency preparation method of a hydrangea polysaccharide of the present embodiment comprises the following steps:

[0061] (1) Hydrangea pretreatment: Hydrangea fruit bodies were vacuum-dried at 60°C to constant weight, crushed, and passed through 60 meshes for later use.

[0062] (2) Get the pretreated hydrangea powder, add 10vol% dimethyl sulfoxide aqueous solution of 30 times the weight, and place it in a bipolar square wave high-voltage pulsed electric field for extraction at a temperature of 40°C, wherein the pulsed electric field strength is 40Kv / cm, the frequency is 800Hz, the treatment time is 1.5h, and the extract is collected.

[0063] (3) The extract was subjected to microwave extraction, the microwave power was 400W, the extraction temperature was 110° C., and the extraction time was 6 min.

[0064] (4) Centrifuge the extract after microwave extraction, concentrate the supernatant to a certain volume under reduced pressure...

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Abstract

The invention discloses an efficient preparation method of Sparassis crispa polysaccharides. The method comprises the following steps: S1, drying and crushing Sparassis crispa sporocarp for later use;S2, taking the pretreated Sparassis crispa powder, adding an aqueous dimethyl sulfoxide solution, extracting in a bipolar square-wave high-voltage pulsed electric field with the pulsed electric fieldintensity of 10-50 Kv / cm and the frequency of 200-1000 Hz, and collecting the obtained extract; S3, carrying out microwave extraction on the extract under a microwave power of 300-500 W at an extraction temperature of 100-120 DEG C; and S4, centrifuging the extract subjected to microwave extraction, carrying out reduced pressure concentration on the obtained supernatant, carrying out alcohol precipitation by using anhydrous ethanol to obtain a crude polysaccharide precipitate, washing the crude polysaccharide precipitate with an organic solvent, and drying to obtain the product. The high-voltage pulsed electric field is combined with microwave extraction, and the dimethyl sulfoxide solution is used as an extraction solvent, so that the Sparassis crispa polysaccharides can be efficiently extracted, and the extraction rate and purity of the Sparassis crispa polysaccharides are improved.

Description

technical field [0001] The invention relates to the technical field of hydrangea extraction, in particular to a high-efficiency preparation method of hydrangea polysaccharide. Background technique [0002] Hydrangea (Sparassis Crispa) is a precious medicinal and edible fungus, enjoying the reputation of "King of Mushrooms". The active ingredients mainly include protein, polysaccharide, ergosterol, glucosylceramide, adenosine and other Some small molecular compounds, etc.; hydrangea contains a large amount of β-glucan, antioxidant substances, vitamins and minerals, and its superoxide dismutase and vitamin E content ranks in the forefront of bacteria and algae food, which has the functions of improving immunity, anti-tumor , antihypertensive, antidiabetic and other pharmacological activities. The main active substances in Hydrangea are polysaccharides, especially Hydrangea β-glucan has the highest content. Natural β-glucan mainly exists in two forms: one is β-(1→3)-glucan, a...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0003
Inventor 吕国英张作法王富根苗立祥宋婷婷
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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