Method for constructing a mouse model of an action mechanism in hypoxic blood-testis-barrier permeability

Abstract

The invention belongs to the technical field of hypoxic blood-testis-barrier permeability action mechanisms, and discloses a method for constructing a mouse model of an action mechanism in hypoxic blood-testis-barrier permeability. The method comprises the following steps: establishing a hypoxic induced C57BL / 6 and testicular mTOR single-gene knockout mouse hypoxic blood-testis-barrier permeability model, separating and collecting mTOR gene knockout mouse and C57BL / 6 mouse seminiferous tubule primary support cell lines and mouse support cell strains, and applying technologies like western blot, immunocytochemistry and qPCR in vitro to prove a potential molecular mechanism of the regulating effect of mTOR on JAM-B. According to the invention, the protective effect of a hypoxic inhibitory activated metabolic balance marker molecule, namely the mTOR in hypoxic blood-testis-barrier permeability and the molecular mechanism of the mTOR on expression of activated JAM-B are defined; and a theoretical basis and a treatment strategy are provided for treatment of the hypoxic blood-testis-barrier permeability.

Description

technical field [0001] The invention belongs to the technical field of the mechanism of hypoxic blood-testis barrier permeability, and in particular relates to a method for constructing a mouse model of the mechanism of action in hypoxic blood-testis barrier permeability. Background technique [0002] At present, the closest prior art: In recent years, more and more people have migrated along the Qinghai-Tibet Railway, and the plateau anoxic (hypoxic hypoxia) environment has a significant negative impact on the reproductive function of male immigrants. A large number of studies on human and rat Decreased spermatogenesis has been reported. The above data only show that hypoxia causes hypoxic spermatogenesis reduction (hypoxicspermatogenesis reduction, HSR), but the specific mechanism is not clear. The blood-testis-barrier (Blood-Testis-Barrier, BTB) between Sertoli cells is an important barrier for spermatogenesis, in which the tight junction (Tight junction, TJ) molecules a...

AI Technical Summary

Problems solved by technology

[0006] In summary, the existing problems in the prior art are: the specific mechanism of hypoxic spermatogenesis reduction (hypoxic spermatogenesis reduction, HSR) in the prior art is not clear, and the pathogenesis of hypoxic spermatogenesis reduction The reason is unknown, and the relevant literature on the role of mTOR in it has not been reported

[0007] The difficulty of solving the above technical problems: mTOR knockout mice have low fecundity, and it takes a long time to cultivate enough mTOR knockout mice to carry out experiments

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