Primer probe system, kit and method for detecting common pathogenic bacteria of severe pneumonia

A primer probe and severe pneumonia technology, applied in the field of molecular biology, can solve problems such as time-consuming, high risk of operation, and restricting the effect of clinical diagnosis and treatment, and achieve the effect of reasonable probe design and dynamic monitoring function

Pending Publication Date: 2020-05-01
MEI HOSPITAL UNIV OF CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the increase of infectious diseases and the aggravation of the severity of the disease, these methods are time-consuming and hig

Method used

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  • Primer probe system, kit and method for detecting common pathogenic bacteria of severe pneumonia
  • Primer probe system, kit and method for detecting common pathogenic bacteria of severe pneumonia
  • Primer probe system, kit and method for detecting common pathogenic bacteria of severe pneumonia

Examples

Experimental program
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Example Embodiment

[0043] Example 1

[0044] In this example, the QAIGEN-55114 kit was used to extract free nucleic acid from clinical samples such as plasma, and then the droplet multicolor digital PCR system was used to detect the type and quantity of pathogenic bacteria in the free nucleic acid of the sample.

[0045] 1. Experimental materials:

[0046] 1. Sample requirements: plasma separated after centrifugation (samples used within 24 hours are stored at 4°C, samples used within 1 month are stored at -20°C, samples used within 3 months are stored at -80°C; plasma cannot be used for more than 3 months To extract cfDNA).

[0047] 2. The equipment and consumables are shown in Table 1

[0048] Table 1 Instrument and equipment table

[0049]

[0050]

[0051] 3. Other reagents and consumables

[0052] 3.1. Reagents: absolute ethanol, analytical pure isopropanol, etc.

[0053] 3.2. Consumables: RNase-free centrifuge tube, DNase-free centrifuge tube, sterile centrifuge tube, low adsorption centrifuge tube, e...

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Abstract

The invention relates to the field of molecular biology, in particular to a primer probe system, kit and method for detecting common pathogenic bacteria of severe pneumonia. Firstly, the invention discloses the primer probe system for detecting the common pathogenic bacteria of the severe pneumonia. The primer probe system for detecting the common pathogenic bacteria of the severe pneumonia comprises a nucleotide sequence group with nucleotide sequences shown in SEQ ID NO: 1-24. The invention further discloses the kit comprising the primer probe system and the method for detecting the pathogenic bacteria by using the primer probe system or the kit. The kit can quickly, efficiently and sensitively detect the common pathogenic bacteria in various biological samples of patients suffering fromthe severe pneumonia. The primer probe system, kit and method provide technical support for quick diagnosis of clinical infection and further help clinicians to achieve precise medical treatment on clinical patients.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a primer-probe system, kit and method for detecting common pathogens of severe pneumonia. Background technique [0002] Severe pneumonia (SP), also known as toxic pneumonia or fulminant pneumonia, can occur in community-acquired pneumonia (community-acquired pneumonia). acquiredpneumonia, CAP), can also occur in hospital acquired pneumonia (hospitalacquiredpneumonia, HAP). Pulmonary parenchymal inflammation caused by various pathogens (bacteria, fungi, viruses, mycoplasma, chlamydia and other microorganisms), resulting in severe bacteremia or toxemia, and then causing blood pressure drop, shock, confusion, irritability, delirium It is a disease that seriously endangers human health, even life-threatening. [0003] The main criteria for the diagnosis of severe pneumonia are: 1. The need for mechanical ventilation; 2. Septic shock requiring the use of vasopressor drugs. The seco...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6851C12Q1/686C12Q1/14C12Q1/10C12Q1/04C12N15/11C12R1/46C12R1/21C12R1/445C12R1/19C12R1/22C12R1/385C12R1/01
CPCC12Q1/689C12Q1/6851C12Q1/686C12Q2600/16C12Q2531/113C12Q2537/143C12Q2563/159C12Q2563/107
Inventor 张顺蔡挺黄左安熊资林静朱留伟董德坤夏江
Owner MEI HOSPITAL UNIV OF CHINESE ACAD OF SCI
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