Method for reducing browning and improving genetic transformation rate of agrobacterium-mediated castor

An Agrobacterium-mediated, transformation-rate technology, which is applied in the agricultural field, can solve problems such as patent publications that have not yet been found, and achieve the effects of rich nutrients such as protein, convenient use, and high fibrosis and cellulose content

Inactive Publication Date: 2020-05-05
TIANJIN UNIV OF SCI & TECH
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for reducing browning and improving genetic transformation rate of agrobacterium-mediated castor
  • Method for reducing browning and improving genetic transformation rate of agrobacterium-mediated castor
  • Method for reducing browning and improving genetic transformation rate of agrobacterium-mediated castor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0048] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0049] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0050] A method for reducing browning and increasing Agrobacterium-mediated castor genetic transformation rate. In the method, in the selection of explants, seed pre-cultivation is used to obtain growing embryos, and the pre-cultivated embryos are peeled off for further pre-cultivation to obtain enlarged mature embryos. , and then take hypocotyls from the expanded and mature embryos as explants; in the method, sodium thiosulfate is used to prevent the explants from browning.

[0051] Preferably, the addition mode of d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for reducing browning and improving the genetic transformation rate of agrobacterium-mediated castor. The method comprises the following steps: in the selection of explants, pre-culturing seeds to obtain growth embryos, stripping the pre-cultured embryos, further carrying out pre-culturing to obtain expanded mature embryos, and taking embryonic axes from the expanded mature embryos as the explants. In the method, sodium thiosulfate is added to prevent the explants from browning. By the method, the browning rate is reduced and the genetic transformation rate ofthe castor is improved by pre-culturing the explants, changing the concentration of the added sodium thiosulfate, using the method and other technologies.

Description

technical field [0001] The invention belongs to the technical field of agriculture, in particular to a method for reducing browning and increasing the genetic transformation rate of castor mediated by Agrobacterium. Background technique [0002] Castor, also known as kenaf, hempseed, etc., is one of the top ten oil crops in the world. Castor bean's high oil content and unique oil composition have become widely valued bioenergy plants and characteristic fine chemical raw materials, widely used in aviation, aerospace, automobiles and high-speed machine tools (biological lubricants), textile printing and dyeing, pesticide auxiliaries , pharmaceuticals and other fields. China is a country with large castor planting area and castor oil consumption. However, due to the lack of castor varieties with wide adaptability, the yield per unit area of ​​castor is uneven, and the planted castor beans cannot meet the needs. (Oil) still needs to be imported. The speed of traditional hybri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H4/00A01H5/00A01H6/38
CPCC12N15/8205A01H4/001A01H4/008
Inventor 王昌禄孙春燕侯宇奇沈国新李贞景
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap