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Method for reducing browning and improving genetic transformation rate of agrobacterium-mediated castor

An Agrobacterium-mediated, transformation-rate technology, which is applied in the agricultural field, can solve problems such as patent publications that have not yet been found, and achieve the effects of rich nutrients such as protein, convenient use, and high fibrosis and cellulose content

Inactive Publication Date: 2020-05-05
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Method for reducing browning and improving genetic transformation rate of agrobacterium-mediated castor
  • Method for reducing browning and improving genetic transformation rate of agrobacterium-mediated castor
  • Method for reducing browning and improving genetic transformation rate of agrobacterium-mediated castor

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Embodiment Construction

[0048] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0049] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0050] A method for reducing browning and increasing Agrobacterium-mediated castor genetic transformation rate. In the method, in the selection of explants, seed pre-cultivation is used to obtain growing embryos, and the pre-cultivated embryos are peeled off for further pre-cultivation to obtain enlarged mature embryos. , and then take hypocotyls from the expanded and mature embryos as explants; in the method, sodium thiosulfate is used to prevent the explants from browning.

[0051] Preferably, the addition mode of d...

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Abstract

The invention relates to a method for reducing browning and improving the genetic transformation rate of agrobacterium-mediated castor. The method comprises the following steps: in the selection of explants, pre-culturing seeds to obtain growth embryos, stripping the pre-cultured embryos, further carrying out pre-culturing to obtain expanded mature embryos, and taking embryonic axes from the expanded mature embryos as the explants. In the method, sodium thiosulfate is added to prevent the explants from browning. By the method, the browning rate is reduced and the genetic transformation rate ofthe castor is improved by pre-culturing the explants, changing the concentration of the added sodium thiosulfate, using the method and other technologies.

Description

technical field [0001] The invention belongs to the technical field of agriculture, in particular to a method for reducing browning and increasing the genetic transformation rate of castor mediated by Agrobacterium. Background technique [0002] Castor, also known as kenaf, hempseed, etc., is one of the top ten oil crops in the world. Castor bean's high oil content and unique oil composition have become widely valued bioenergy plants and characteristic fine chemical raw materials, widely used in aviation, aerospace, automobiles and high-speed machine tools (biological lubricants), textile printing and dyeing, pesticide auxiliaries , pharmaceuticals and other fields. China is a country with large castor planting area and castor oil consumption. However, due to the lack of castor varieties with wide adaptability, the yield per unit area of ​​castor is uneven, and the planted castor beans cannot meet the needs. (Oil) still needs to be imported. The speed of traditional hybri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H4/00A01H5/00A01H6/38
CPCC12N15/8205A01H4/001A01H4/008
Inventor 王昌禄孙春燕侯宇奇沈国新李贞景
Owner TIANJIN UNIV OF SCI & TECH
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