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Method for leukaemia high-flux medicine screening

A leukemia, high-throughput technology, applied in the field of high-throughput drug screening for leukemia, can solve the problems of patient recurrence and no breakthrough in curative effect, achieve good curative effect, increase drug curative effect, and shorten the time

Pending Publication Date: 2020-05-08
HARVEST BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current treatment can make most patients achieve complete remission, there is still no breakthrough in its curative effect, and about 70% of patients still relapse

Method used

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  • Method for leukaemia high-flux medicine screening
  • Method for leukaemia high-flux medicine screening
  • Method for leukaemia high-flux medicine screening

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0037] Patient, female 30 years old

[0038] Clinical diagnosis: Leukemia-like reaction, leukocytes increase significantly after severe infection, similar to leukemia blood picture

[0039] Screening results: Drug screening results recommend the following drugs: daunorubicin, mitoxantrone, homoharringtonine, Dinaciclib, cinobufacin. Among them, Dinaciclib is an effective cyclin-dependent kinase (CDK) inhibitor.

[0040]

[0041]

[0042] Inhibition rate (%): in the high-throughput drug in vitro test, the killing effect of the drug screening dose on the patient's tumor cells.

[0043] Inhibition rate (%)=(1-survival number of tumor cells in drug screening group / survival number of tumor cells in blank control group)*100%

example 2

[0045] Patient male 18 years old

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PUM

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Abstract

The invention provides a method for leukaemia high-flux medicine screening. The method comprises the following steps of performing separation, extraction, identification and culture on neoplastic hematologic disorder stem cells: performing acquisition on bone marrow samples, performing separation on mononuclear cells, performing separation on leukaemia stem cell groups through an immunomagnetic bead method; and performing subculturing on the cells after separation and purification of magnetic beads; and establishing a medicine library: according to a medicine description and pharmacokinetics,selecting the concentration of medicines, performing mixed culture on the medicines and the neoplastic hematologic disorder stem cells, and analyzing the restraining effect of the medicines on the neoplastic hematologic disorder stem cells. According to the method for leukaemia high-flux medicine screening disclosed by the invention, in accordance with a patient, an appropriate treatment scheme and opinions can be accurately given in an individualizing manner, a valuable treatment opportunity is strived for the recurrent and refractory patient, reagent risk is reduced, the medicine curative effect is increased to the maximum degree, and medicine side effects are reduced to the maximum degree; and besides, pharmaceutical combinations can be performed, in accordance with clinical frequently-used chemotherapeutics combinations, comprehensive marking is performed, and a reference is provided for a doctor in the respect of formulating clinical united medication for the patient.

Description

technical field [0001] The present invention relates to a method for high-throughput drug screening for leukemia. Background technique [0002] John Dick isolated and purified CD34 in acute myeloid leukemia (AML) patients + / CD38 - phenotype of AML cells, inoculated into NOD / SCID mice, can produce human-like leukemia, and CD34 - / CD38 + Leukemia cells of the phenotype are less likely to form tumors, so it is speculated that CD34 + / CD38 - The phenotypic leukemia cells are AML stem cells. After entering the 21st century, people have also isolated and verified cancer stem cells in solid tumors (the earliest in breast and brain tumors), completely heralding the arrival of the era of cancer stem cells. Cancer stem cells refer to tumor cells with stem cell characteristics, which have the ability of "self-renewal" and "multi-directional differentiation". Usually, such cells are considered to have the potential to form tumors and develop into cancer, especially after the canc...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5011
Inventor 周碧柳陆益李均翔董云加俞剑锋沈丽霞
Owner HARVEST BIOTECH CO LTD
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