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Application and method of IL-1a in establishing human gingival fibroblast oral anti-inflammatory model

A fibroblast and inflammation model technology, applied in animal cells, vertebrate cells, cell culture active agents, etc., can solve problems such as loss of efficacy, long delivery period, and poor stability of inflammation models

Pending Publication Date: 2020-05-12
GUANGDONG BOXI BIO TECH CO LTD
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Problems solved by technology

However, these two LPSs have the following two disadvantages in the construction of cell inflammation models: 1. The stability of the inflammation model is poor, and the experiment repetition rate is low, which often leads to conflicting evaluations on the anti-inflammatory effects of the same sample, making it impossible to accurately evaluate the oral cavity. Authenticity of care ingredients and product anti-inflammatory effects
2. LPS is unstable, relatively expensive, and has a long delivery period, especially Pg.LPS, and there are many disadvantages in the storage method of LPS, such as: the concentration of LPS must be greater than 2mg / ml when preparing, otherwise LPS Long-term storage can adhere to the tube wall, and the effective ingredients will be reduced and lose their effectiveness
The latter, these often bring a lot of inconvenience to experimental operators and enterprises

Method used

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  • Application and method of IL-1a in establishing human gingival fibroblast oral anti-inflammatory model
  • Application and method of IL-1a in establishing human gingival fibroblast oral anti-inflammatory model
  • Application and method of IL-1a in establishing human gingival fibroblast oral anti-inflammatory model

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Experimental program
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Embodiment approach

[0034] (1) Effects of different concentrations of stimulants on the viability of human gingival fibroblasts: set up a blank group, a control group and an experimental group. The following three substances were selected as the stimulus: IL-1a, Ecoli.LPS and Pg.LPS. Among them, three substances are divided into 3-4 concentrations for concentration exploration. The specific concentrations are shown in the table below. IL-1a is set at 1ng / ml, 5ng / ml, 10ng / ml, 15ng / ml; Ecoli.LPS is set at 1ug / ml, 10ug / ml, 50ug / ml, 100ug / ml; Pg.LPS is set at 1ug / ml, 10ug / ml ml, 50ug / ml, 100ug / ml.

[0035] (2) Inflammation model induced by IL-1a in human gingival fibroblasts: set up control group and experimental group. 2×10 human gingival fibroblasts 5 Cells / well were seeded in a 24-well plate, and 2 mL of high-DMEM medium containing 10% FBS was added to each well, and placed at 37±1°C, 5±1% CO 2 , 95% relative humidity incubator, and the cell plating rate reached between 40% and 60% at 24 hour...

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Abstract

The invention provides an application and a method of IL-1a in establishing a human gingival fibroblast oral anti-inflammatory model and the human gingival fibroblast inflammation model. The human gingival fibroblast oral anti-inflammatory model provided by the invention can more comprehensively reflect efficacy of oral care products, and provides a more objective, scientific and accurate evaluation mode for evaluating efficacy of oral anti-aging products.

Description

technical field [0001] The invention belongs to the technical field of detection of safety and efficacy of oral care products, and in particular relates to a method for establishing a human gingival fibroblast inflammation model of IL-1a, an application of IL-1a in establishing a human gingival fibroblast inflammation model, and an IL-1a method for establishing a human gingival fibroblast inflammation model. Inflammation model of human gingival fibroblasts established by -1a. Background technique [0002] Inflammation is a pathological process in which living tissue responds mainly to defense against injury or infection. In the inflammatory process, on the one hand, damage factors can directly and indirectly cause tissue and cell damage, and on the other hand, they can also cause vascular congestion and exudative reactions, which exist in the form of diluting, killing and surrounding damaged tissues. In the past ten years, with the development of biotechnology, when people ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077G01N33/74G01N33/68
CPCC12N5/0656G01N33/74G01N33/6869G01N33/6866C12N2501/2301C12N2500/02C12N2523/00G01N2800/7095G01N2333/72G01N2333/7151G01N2333/7155
Inventor 杨文娟卢永波张勇杰
Owner GUANGDONG BOXI BIO TECH CO LTD
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