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A kind of new bacterial strain of Uchid rugosa and its artificial cultivation method

A technology of artificial cultivation and strains, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of cumbersome cultivation process of fermentation and sterilization, no research reports on strains, unsustainability, etc., and achieves a wide range of suitable growth temperatures, The effect of strong resistance and disease resistance, and short post-ripening period

Active Publication Date: 2022-05-20
鹤灵谷(福州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this patent, there is also a relatively tedious cultivation process of fermentation and sterilization, and at the same time, alkaline lime is added to the fungus stick culture medium, which has the same problem as the above-mentioned "a method for cultivating the fruiting body of Wuzhi fruiting body". , In addition, the patent also uses acacia wood pellets as the main raw material. However, acacia trees are basically not economic forests, but mostly ecological protection forests, natural forests and scenic forests. Cultivating Uzhi with such raw materials is bound to be unenvironmental and unsustainable Yes, and the cost will be high
[0006] This application is a new fungus strain of Uchid Uchidis collected under the acacia forest in Taiwan Province, Jianxin Town, Cangshan District, Fuzhou City, and has been isolated and domesticated. After research, there is no research report on this strain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A cultivation method suitable for the new Uzhi XLZ001 strain and other existing Uzhi strains, including tissue separation and purification to obtain the strain, preparation of the mother seed, preparation and cultivation of the solid original seed, and management of the fruiting. The specific steps are as follows:

[0039] (1) Bacteria obtained by tissue separation and purification: Wash the wild fresh fruiting bodies of Uzhis chinensis with sterile water, cut off the internal tissue from the split surface with a scalpel, and cut the separated fruiting body tissues of Uzhis chinensis into 0.2cm side lengths The left and right small pieces are connected to the mother seed medium, and the inoculated petri dish is cultivated at a constant temperature of 24°C for 7-10 days to obtain isolated and purified bacteria; the mother seed medium is 13% of potato, 13% by mass percentage, Agar 2%, glucose 2%, fish peptone 0.5%, potassium dihydrogen phosphate 0.2%, the balance is water;...

Embodiment 2

[0049] A cultivation method suitable for the new Uzhi XLZ001 strain and other existing Uzhi strains, including tissue separation and purification to obtain the strain, preparation of the mother seed, preparation and cultivation of the solid original seed, and management of the fruiting. The specific steps are as follows:

[0050] (1) Bacteria obtained by tissue separation and purification: Wash the wild fresh fruiting bodies of Uzhis chinensis with sterile water, cut off the internal tissue from the split surface with a scalpel, and cut the separated fruiting body tissues of Uzhis chinensis into 0.5cm side lengths The left and right small pieces are connected to the mother seed medium, and the inoculated petri dish is cultivated at a constant temperature of 28°C for 7-10 days to obtain isolated and purified strains; the mother seed medium is 18% potato, 18% by mass percentage, Agar 1%, glucose 1%, fish peptone 1%, potassium dihydrogen phosphate 0.1%, the balance is water;

[0...

Embodiment 3

[0060] A cultivation method suitable for the new Uzhi XLZ001 strain and other existing Uzhi strains, including tissue separation and purification to obtain the strain, preparation of the mother seed, preparation and cultivation of the solid original seed, and management of the fruiting. The specific steps are as follows:

[0061](1) Bacteria obtained by tissue separation and purification: Wash the wild fresh fruiting bodies of Uzhis chinensis with sterile water, cut off the internal tissue from the split surface with a scalpel, and cut the separated fruiting body tissues of Uzhis chinensis into 0.4cm side lengths The left and right small pieces are inserted into the mother seed medium, and the inoculated petri dish is cultivated at a constant temperature of 26°C for 8 days to obtain isolated and purified strains; the mother seed medium is 15% potato, 1 agar 1 %, glucose 2%, fish peptone 0.5%, potassium dihydrogen phosphate 0.2%, and the balance is water;

[0062] Among them, w...

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Abstract

The present invention relates to a new bacterial strain of Uzhi, which is Uzhi ( Amauroderma rugosum ) XLZ001, the deposit number is CCTCC NO: M20191076. Uchid ( Amauroderma Rugosum ) XLZ001 artificial cultivation method, including tissue separation and purification to obtain strains, mother seed preparation, solid original seed preparation and cultivation, and management of mushroom production; The production application conforms to the market demand.

Description

technical field [0001] The invention relates to a ganoderma fungus strain, in particular to a new Uzhi fungus strain and an artificial cultivation method. Background technique [0002] Ganoderma lucidum has a long history of medicinal use in my country, and has long been used as an adjuvant therapy for bronchitis, hepatitis, hypertension, tumors, immune disorders and other diseases. Modern pharmacological and clinical research results also show that Ganoderma lucidum contains anti-tumor and immune-regulating effects active ingredient. Since Ganodermaceae was established in 1984, there have been more than 200 species of large-scale fungi of Ganodermaceae reported in the world. There are 103 species of Ganoderma recorded in my country, 14 of which have been used by people. Ganoderma lucidum. [0003] Uchid ( Amauroderma rugosum ), also known as Black False Mushroom, belongs to the Phylum Fungi, Phyllomycetes, Phyllomycetes, Ganodermaceae, Ganoderma, because its young fruiting...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G18/00A01G18/40A01G18/20A01H15/00C12R1/645
CPCA01G18/00A01G18/40A01G18/20A01H15/00C12R2001/645C12N1/145
Inventor 陈丽丽陈靖思李治君马云龙邵帅郭琳
Owner 鹤灵谷(福州)生物科技有限公司
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