Micro-heterotypic cell-loaded alginic acid gel, and preparation method and application thereof
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A micro-alginate, alginic acid technology, applied in medical science, prosthesis, etc., can solve the problems of inability to obtain shape, uniform micro-alginate cell-carrying sea gel, etc.
Inactive Publication Date: 2020-05-26
GUANGDONG UNIV OF TECH
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Problems solved by technology
[0005] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of the existing micro-shaped cell-loaded sea gel that cannot be obtained with a uniform shape and without damaging the internal cells, and to provide a micro-shaped cell-loaded alginate gel
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Embodiment 1
[0052] A micro-shaped cell-loaded alginic acid gel, the micro-shaped cell-loaded alginic acid gel has a four-layer structure, from the inside to the outside: cells and nutrient solution (seed cells such as: embryonic stem cells, pluripotent stem cells, etc., nutrient solution : Amino acids, vitamins, glucose and inorganic salts and other normal components needed for cell growth and proliferation)—inner special-shaped gel—drug solution, such as epidermal growth factors, fibroblast growth factors, etc., choose different drugs according to needs—outer The inner gel is rod-shaped, and the outer gel is spherical.
[0053] Among them, the inner heterogeneous gel provides a biological scaffold for the directional propagation of cells; the drug solution causes the cells to functionally differentiate and form cell groups of specific tissues / organs; the outer gel ensures the relative independence of the internal substances. Since the gel is degradable, after the internal cells are cultu...
Embodiment 2
[0072] A micro-shaped cell-loaded alginic acid gel, the micro-shaped cell-loaded alginic acid gel has a four-layer structure, from the inside to the outside: cells and nutrient solution-inner special-shaped gel-drug solution-outer gel, The inner special-shaped gel is rod-shaped, and the outer gel is spherical.
[0074] Nutrient solution: Amino acids, vitamins, glucose, and inorganic salts are the normal ingredients needed for cells to produce and proliferate.
[0075] The specific preparation method is as follows:
[0076] S1. Preparation of inner layer heterogeneous gel: the solution containing seed cells and gel material is used as the inner phase, and the oil phase with certain biocompatibility is used as the outer phase, and the two-phase flow is used to generate droplets to form gel droplets. Then the gel droplet is shaped through the flow channel, and at the same time the gel droplet itsel...
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Abstract
The invention discloses a micro-heterotypic cell-loaded alginic acid gel, and a preparation method and an application thereof. The micro-heterotypic cell-loaded alginic acid gel is of a four-layer structure and sequentially comprises cells and a nutrient solution, inner-layer heterotypic gel, a drug solution and outer-layer gel from inside to outside. The inner-layer heterotypic gel of the microheterotypic cell-loaded alginic acid gel provides a biological scaffold for directional propagation of cells; the drug solution enables the cells to generate functional differentiation to form a cell population of specific tissues / organs; and the outer-layer gel ensures the relative independence of internal substances. According to the preparation method , the micro-special-shaped carrier cell gel with good uniformity can be rapidly formed in a large flux, the cell-loaded gel with different functions can be prepared by changing cell types, cell density and medicines contained in a water phase, the cell-loaded gel can be widely applied to transplantation of various engineering organs and repair of damaged organs and tissues, and different functional requirements of the engineering organs aremet.
Description
technical field [0001] The present invention relates to the field of hydrogel technology, more specifically, to a micro-shaped cell-loaded alginic acid gel and its preparation method and application. Background technique [0002] Gel refers to the colloidal particles or polymers in the sol or solution linking to each other under certain conditions to form a gel with a three-dimensional network structure, and the gaps in its structure are filled with liquid as a dispersion medium. There are many kinds of gels, such as glucomannan gum, gel candy, aloe vera gel, airgel, etc. Among them, sodium alginate is used because of its good biocompatibility, non-toxicity and biodegradability. The prepared alginic acid hydrogel has been widely used in food industry and medicine. The preparation of the gel is divided into physical cross-linking and chemical cross-linking. Physical crosslinking such as heating, diffusion, etc.; chemical crosslinking such as ultraviolet crosslinking, etc. ...
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