Method capable of simultaneously detecting plurality of genotypes of hepatitis E viruses

A technology of hepatitis E virus and genotype, applied in the field of detecting multiple genotypes of hepatitis E virus at the same time, can solve the problems of human health threats, few detection methods of HEVtype3 virus HEVtype4 virus, lack of epidemic prevention, etc.

Pending Publication Date: 2020-06-05
智享生物(苏州)有限公司
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Problems solved by technology

[0004] At present, the clinical diagnosis of HEV is mainly to detect whether the IgM antibody of HEV has been produced in the patient's blood, and the current methods are all for HEV type I virus, and there are few detection methods for HEV type 3 virus and HEV type 4 virus In this way, there will be deficiencies in epidemic prevention, which will pose a great threat to human health

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  • Method capable of simultaneously detecting plurality of genotypes of hepatitis E viruses
  • Method capable of simultaneously detecting plurality of genotypes of hepatitis E viruses

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Embodiment 1

[0043] A method capable of simultaneously detecting multiple genotypes of hepatitis E virus, targeting type 1 genotype hepatitis E virus, type 2 genotype hepatitis E virus, type 3 genotype hepatitis E virus, type 4 genotype hepatitis E Hepatitis virus gene sequence, comprehensive comparison of gene sequence, design of primers and probes;

[0044] Wherein, the specific steps of designing primers and probes are:

[0045] 1) Collect the gene sequences of different types of HEV, and find out the regions with high similarity of different types of genes through multi-gene comparison method, and design primers and probes in this region;

[0046] 2) Backtrack the designed primers and probes to similar regions, avoid parts with low similarity in the region, and select fragments in the PCR reaction that are smaller than 200bp and whose dissociation temperature of primers and probes is greater than 60°C; And carry out the transformation of the shared base of the primers, and design diff...

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Abstract

The invention discloses a method capable of simultaneously detecting a plurality of genotypes of hepatitis E viruses. The method comprises the following steps: aiming at the genetic sequences of genotype-1 hepatitis E viruses, genotype-2 hepatitis E viruses, genotype-3 hepatitis E viruses and genotype-4 hepatitis E viruses, carrying out comprehensive comparison on the genetic sequences, and designing primers and probes, wherein each group of the probes is designed for different types of viruses, and can be used for detecting type-1 HEV, type-3 HEV or type-4 HEV, and type-1, type-2, type-3 andtype-4 HEV. Thus, different requirements can be met.

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to a method capable of simultaneously detecting multiple genotypes of hepatitis E virus. Background technique: [0002] Hepatitis E virus (HEV) is a serious viral infectious disease all over the world. Every year, an estimated 20 million people worldwide are infected with HEV, resulting in about 3.3 million people with symptomatic HEV. According to WHO statistics, HEV caused 44,000 deaths in 2015, accounting for about 3.3% of the mortality rate of viral hepatitis. The disease is mainly transmitted by the fecal-oral route and is mainly found in East and South Asia. [0003] HEV currently has at least four different genotypes. The first and second types only infect humans, while the third and fourth types have latent viruses in pigs and deer, and occasionally infect humans. The virus is transmitted to humans through feces, such as drinking contaminated drinking water, and infection thr...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12R1/93
CPCC12Q1/707C12Q1/6851C12Q2531/113
Inventor 李智
Owner 智享生物(苏州)有限公司
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