Autoantibody marker for distinguishing hepatocellular carcinoma from normal people and screening method thereof
A technology for hepatocellular carcinoma and autoantibodies, which is applied in the analysis of materials, biological tests, and material inspection products, can solve problems such as non-specificity, achieve the effects of avoiding defects, changing liver cancer diagnosis and curative effect monitoring, and improving clinical diagnostic capabilities
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[0031] 557 cases of hepatocellular carcinoma patients and 343 healthy controls were detected using a small protein chip containing 100 recombinant proteins that focused on hepatocellular carcinoma. Through comparison and analysis of the response data, it was finally determined that 16 had certain distinguishing ability or combined discrimination Candidate autoantibody markers of competence.
[0032] 1. Reagents needed for experiment
[0033] 1) Blocking solution: 3ml 10% BSA, add 7ml 1x PBS solution, mix well, and place on ice.
[0034] 2) Incubation solution: 1ml 10% BSA, add 9ml 1x PBST solution, mix well, and place on ice.
[0035] 3) Washing solution: 1x PBST, stored in a refrigerator at 4°C.
[0036] 2. Experimental operation steps
[0037] 1) Rewarming: Take the chip out of the -80°C refrigerator, reheat it in the 4°C refrigerator for half an hour, and then reheat it at room temperature for 15 minutes;
[0038] 2) Sealing: After rewarming the chip, fix the 14blocks fence. After fix...
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