Autoantibody marker for distinguishing hepatocellular carcinoma from normal people and screening method thereof

A technology for hepatocellular carcinoma and autoantibodies, which is applied in the analysis of materials, biological tests, and material inspection products, can solve problems such as non-specificity, achieve the effects of avoiding defects, changing liver cancer diagnosis and curative effect monitoring, and improving clinical diagnostic capabilities

Pending Publication Date: 2020-06-05
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that there are some tumor-specific antigens, but no specificity among different tumors, such as p53; there are also some tumor-specific antigens, such as many antigens in prostate cancer are oxidative stress-related proteins

Method used

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  • Autoantibody marker for distinguishing hepatocellular carcinoma from normal people and screening method thereof
  • Autoantibody marker for distinguishing hepatocellular carcinoma from normal people and screening method thereof
  • Autoantibody marker for distinguishing hepatocellular carcinoma from normal people and screening method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 557 cases of hepatocellular carcinoma patients and 343 healthy controls were detected using a small protein chip containing 100 recombinant proteins that focused on hepatocellular carcinoma. Through comparison and analysis of the response data, it was finally determined that 16 had certain distinguishing ability or combined discrimination Candidate autoantibody markers of competence.

[0032] 1. Reagents needed for experiment

[0033] 1) Blocking solution: 3ml 10% BSA, add 7ml 1x PBS solution, mix well, and place on ice.

[0034] 2) Incubation solution: 1ml 10% BSA, add 9ml 1x PBST solution, mix well, and place on ice.

[0035] 3) Washing solution: 1x PBST, stored in a refrigerator at 4°C.

[0036] 2. Experimental operation steps

[0037] 1) Rewarming: Take the chip out of the -80°C refrigerator, reheat it in the 4°C refrigerator for half an hour, and then reheat it at room temperature for 15 minutes;

[0038] 2) Sealing: After rewarming the chip, fix the 14blocks fence. After fix...

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Abstract

The invention discloses an autoantibody marker for distinguishing hepatocellular carcinoma from normal people and a screening method thereof. According to the invention, a protein microchip (HCC Focused Arrays) which contains 100 recombinant proteins and focuses on hepatocellular carcinoma is adopted to carry out contrast detection on hepatocellular carcinoma patients and healthy people; 16 candidate autoantibody markers with certain distinguishing capability or combined distinguishing capability are determined through responsive data comparison and analysis. The autoantibody marker and the screening method are helpful for changing the current situation of liver cancer diagnosis and curative effect monitoring, and haveimportant clinical value for liver cancer diagnosis.

Description

Technical field [0001] The invention relates to the field of biomedicine, and is connected with applied basic medical research and clinical research; covering oncology, diagnostics and immunology. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the common malignant tumors. It has hidden onset, high degree of malignancy, and high mortality. Therefore, timely and accurate diagnosis is essential to improve the survival rate of patients. Currently, Alpha fetoprotein (AFP) combined with imaging and pathological examinations is mainly used clinically for early diagnosis of liver cancer; however, the specificity and sensitivity of AFP for liver cancer screening are not very ideal. With the continuous development of molecular biology, a variety of new markers have been discovered, especially the diagnostic value of autoantibodies. [0003] Autoantibodies are specific antibodies produced by the body in an immune response to abnormal tumor antigens. They have the foll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/564G01N33/574G01N33/68
CPCG01N33/564G01N33/57438G01N33/57484G01N33/6854
Inventor 张舒高强毕利军李阳刘羽鸣杨思贤刘诚喜董良庆
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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