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Group of markers for early diagnosis of abortion caused by prethrombotic state and application thereof

A technology of early diagnosis and markers, applied in disease diagnosis, biological testing, biomaterial analysis, etc., can solve problems such as miscarriage and affecting embryo growth and development

Pending Publication Date: 2020-06-09
BEIJING OBSTETRICS & GYNECOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The inventors have screened two specific proteins, IGFBP-rPl and VEGF, through protein chip technology in the previous study on the correlation between recurrent abortion prothrombotic state and kidney deficiency and blood stasis. Among them, IGFBP-rPl, as a secreted protein, may inhibit The EMT process affects the growth and development of the embryo, leading to miscarriage; VEGF may cause vasoconstriction through the distribution of its receptor KDR protein, promote platelet aggregation, form a prethrombotic state, and eventually lead to miscarriage

Method used

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  • Group of markers for early diagnosis of abortion caused by prethrombotic state and application thereof
  • Group of markers for early diagnosis of abortion caused by prethrombotic state and application thereof
  • Group of markers for early diagnosis of abortion caused by prethrombotic state and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Differential protein screening

[0036] 1. Clinical data

[0037] 1.1 Case source:

[0038] 1.2 Case grouping: 12 serum samples from women with normal pregnancy history (normal group), 24 serum samples from recurrent abortion patients caused by prethrombotic state (prethrombotic state group), serum samples from recurrent abortion patients not caused by prethrombotic state Samples of 12 cases (non-thrombotic state group).

[0039] 1.3 Number of cases:

[0040] Protein microarray detection: 12 cases in the normal group, 24 cases in the prethrombotic state group, and 12 cases in the non-prothrombotic state group.

[0041] 2. Materials and instruments used in the present invention

[0042] 2.1 Kit: QAH-CAA-440 kit, kit contains:

[0043] The kit is stored at -20°C. After starting to use, slide chips, cytokine standard mixed powder, detection antibody mixture, Cy3-streptavidin should be stored at -20°C, and other reagents should be stored at 4°C to avoid repea...

Embodiment 2

[0113] Example 2 Differential Protein Verification

[0114] 1. The verification of the differential protein between the non-prothrombotic state group, the prethrombotic state group and the normal group was verified by the protein chip method, refer to Example 1.

[0115] In the results of the preliminary screening, the inventor found that 38 proteins were significantly up-regulated or down-regulated in the disease group compared with the normal group. In order to further confirm the differential expression of these proteins, the inventor selected 20 proteins for verification. The samples tested were 58 cases in the prethrombotic state group, 34 cases in the non-thrombotic state group, and 25 cases in the normal group.

[0116] The results showed that in the verified results, 10 proteins were differentially expressed in the disease group compared with the normal group, 4 of which were up-regulated and 6 were down-regulated, as shown in Table 7.

[0117] In addition, the result...

Embodiment 3

[0127] Embodiment 3 receiver operating characteristic (ROC) curve analysis

[0128] A receiver operating characteristic (ROC) curve was constructed to compare the diagnostic ability of 33 serum markers in distinguishing recurrent abortion patients caused by prothrombotic state from healthy controls. Due to the large number, the ROC curves of six serum markers are given as reference examples, such as Figure 9 shown. Under the optimal cutoff value, the sensitivity of the 33 serum markers was between 0.621-0.920, the specificity was between 0.675-0.948 and the AUC value was between 0.688-0.841.

[0129] The combined AUC of these 33 serum markers can reach 0.948, and the sensitivity and specificity are 1.00 and 0.828 respectively (such as Figure 10 shown). These results indicated that, compared with 33 individual protein markers of prothrombotic state induced abortion, the combination of 33 markers had higher sensitivity and specificity for the early detection of prothromboti...

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Abstract

The invention discloses a group of markers for diagnosing abortion caused by a prethrombotic state and application thereof. The markers are composed of the following proteins: Prolactin, MMP-3, Testican 2, hCGb, IGF-1R, SLAM, Furin, IL-17C, APRIL, B2M, Syndecan-1, JAM-B, Gas 1, VEGF R1, Desmoglein 2, CEA, BLC, G-CSF R, ANG-2, Syndecan-3, MIF, MCP-4, Follistatin-like 1, MCP-3, NRG1-b1, EGF, CA15-3,VEGF R3, IL-17, BMP-4, S100A8, GROa, and ADAM8. Experiments prove that the protein shows significant differential expression between a patient suffering from abortion caused by the prethrombotic state and a normal person, and it is prompted that the proteins can be used as a diagnostic marker for abortion caused by the prethrombotic state for the diagnosis of abortion caused by the prethromboticstate.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a group of markers for early diagnosis of abortion caused by prethrombotic state and application thereof. Background technique [0002] There are about 35,000 genes in humans, but there are more than 100,000 proteins in the human body. A gene does not express only one protein. To study life phenomena and explain the laws of life activities, it is not enough to only understand the structure of the genome. It is necessary to have a deeper understanding of the importance of proteins, the direct executors of life activities. Therefore, as an important content of functional genomics One of the proteomics (Proteomics) came into being. The concept of "proteome" was proposed by Wilkins and WilliamS of Australia in 1994. It is defined as all the corresponding proteins expressed by the genome of a cell or a tissue. It is a whole composed of all proteins corresponding to a gen...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/689G01N2800/36Y02A50/30
Inventor 阴赪宏武颖何军琴辛明蔚王景尚
Owner BEIJING OBSTETRICS & GYNECOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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