Composition for treating lung tumors

A composition and lung tumor technology, applied in the field of medicine, can solve the problems affecting the chemotherapy effect of non-small cell lung cancer, drug resistance, non-small cell lung cancer resistance to chemotherapeutic drugs, etc., and achieve enhanced anti-tumor effect and drug taste. Less and better for quality control

Active Publication Date: 2020-06-12
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cisplatin is one of the most commonly used chemotherapeutic drugs for the treatment of lung cancer. Clinical studies have shown that cisplatin can indeed prolong the survival of patients. Both the second-generation cisplatin and the third-generation cisplatin have drug resistance, which has become a major obstacle affecting the chemotherapy effect of non-small cell lung cancer

Method used

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  • Composition for treating lung tumors
  • Composition for treating lung tumors
  • Composition for treating lung tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Inhibitory effect of the combination drug of the present invention on non-small cell lung cancer cells in vitro

[0023] 1. The specific use method of the experimental drug

[0024] (1) Bat Gesulindine drug: dissolved in DMSO solution, prepared as a 10mM stock solution, stored in a -20°C refrigerator; when used, diluted with complete medium to a 5μM working solution.

[0025] (2) Cisplatin drug: dissolved in DMSO solution, made into a 10mM stock solution, stored in a -20°C refrigerator; used by diluting it into a corresponding working solution with complete medium.

[0026] 2. Experimental method:

[0027] 2.1CCK-8 experiment

[0028] Inoculate non-small cell lung cancer cells (A549 or NCI-H1299 cell line) in a culture dish, divide the cells into multiple test groups according to Table 1, and set 3-6 duplicate holes in each group (the cell density is about 8000 / wells); and set up a cell-free group, filled with sterile PBS.

[0029] Table 1 The final conce...

Embodiment 2

[0052] Embodiment 2 animal experiments

[0053] 1. The specific use method of the experimental drug

[0054] (1) Bat Gesulindine drug: Dissolve in DMSO solution to prepare 20mg / ml stock solution (stored in -20°C / 4°C refrigerator), and dilute it with normal saline to make 2mg / mL working solution when in use.

[0055] (2) Cisplatin drug: Dissolve in DMSO solution to prepare 0.8 mg / ml stock solution (stored in -20°C / 4°C refrigerator), and dilute it with normal saline to make 0.08 mg / mL working solution.

[0056] 2. Experimental method:

[0057] 2.1 Construction of xenograft tumor model

[0058] Nude mice were selected as SPF grade BALB / c-nu / nu, 6 weeks old, male. Non-small cell lung cancer cells (NCI-H1299 cell line) in the logarithmic growth phase were selected to inoculate nude mice at a concentration of 5×10 7 cells / mL, each mouse was subcutaneously inoculated with 100 μL of cell solution. When the tumor volume grows to about 60mm 3 At the same time, the nude mice were r...

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PUM

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Abstract

The invention discloses a composition for treating lung tumors. The composition is prepared from daurisoline and cisplatin; few medicines are used, and quality control is facilitated; the combined application of the dauricine and the cisplatin has a good killing effect on non-small cell lung cancer no matter at the cellular level or the animal level, can induce tumor cell apoptosis and enhance theanti-tumor effect, which indicates that the dauricine can have a synergistic effect on the cisplatin and improve the clinical drug resistance of the cisplatin; and the composition has important significance for treating lung tumors, especially non-small cell lung cancer, and has huge social value and economic value.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a composition, in particular to a composition for treating lung tumors. Background technique [0002] Lung cancer is one of the common causes of human death worldwide, and non-small cell lung cancer (NSCLC) is the most common type of lung cancer, with high morbidity and mortality. In addition, due to environmental and other factors, the incidence of lung cancer is increasing year by year, so it is urgent to explore effective lung cancer treatment strategies. Today, there are three primary treatments for cancer: surgery, chemotherapy, and radiation. Surgery and radiotherapy are usually used to remove tumor cell lesions, while chemotherapy drugs can spread throughout the body and effectively kill tumor cells, so chemotherapy is mainly used in postoperative adjuvant therapy in clinical practice. For patients with advanced cancer, even if it is difficult to eradicate cancer cells, chemotherapy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/243A61K31/4725A61P35/00A61P11/00
CPCA61K33/243A61K31/4725A61P35/00A61P11/00A61K2300/00
Inventor 肖建勇杜标炎王坤占玉娟陈博南
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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