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Biological reaction process on-line cyclic sampling device and sampling method

A biological reaction device and a cycle sampling technology, applied in the direction of sampling devices, etc., can solve the problems of inability to realize online cycle sampling, cumbersome operation, large dead volume, etc., to avoid bacterial contamination, reduce dead volume, and small sampling time interval Effect

Pending Publication Date: 2020-06-16
LUOYANG TMAXTREE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the aseptic sampling operation of the above-mentioned patents is cumbersome, and online cycle sampling cannot be realized. At the same time, the dead volume caused by the realization is relatively large, resulting in waste of sampling.

Method used

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  • Biological reaction process on-line cyclic sampling device and sampling method
  • Biological reaction process on-line cyclic sampling device and sampling method
  • Biological reaction process on-line cyclic sampling device and sampling method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Fermentation medium, strain and system: LB medium, Escherichia coli, 3L LB medium in a 5L tank, culture temperature 37℃.

[0059] The flow rate of the peristaltic pump is 0.5 mL / min; the inner diameter of the connected silicone tube is 0.8mm; the inner diameter of the two multi-channel switching valves is 0.8mm; the connecting pipelines between the multi-channel switching valve and the sample bottle and the waste bottle are organic plastic hard pipe inner diameters It is 0.2mm.

[0060] The sampling volume is 0.5 mL. Set a sampling every 2 h for 24 h. The volume of the 12 samples retained is shown in Table 1:

[0061] Table 1 Results of retention samples

[0062] frequency123456 Sampling volume (mL)0.510.480.50.490.480.49 frequency789101112 Sampling volume (mL)0.500.480.510.480.480.49

[0063] The average value is 0.49 mL, and the standard deviation is 0.011.

Embodiment 2

[0065] Fermentation medium, strains and fermentation system: LB medium, Escherichia coli, 3L LB medium in a 5L tank, culture temperature 37℃.

[0066] The flow rate of the peristaltic pump is 20 mL / min; the inner diameter of the connected silicone tube is 2mm; the inner diameter of the two multi-channel switching valves is 1.5mm; the connecting pipelines between the multi-channel switching valve and the sample bottle and the waste bottle are organic plastic hard tubes with an inner diameter of 1.0mm.

[0067] The sampling volume is set to 15 mL and sampling is performed every 2 h for 24 h. The final volume of the 12 samples taken out is shown in Table 2:

[0068] Table 2 Retained sample results

[0069] frequency123456 Sampling volume (mL)14.8914.9114.8714.8514.9214.89 frequency789101112 Sampling volume (mL)14.9014.8314.8714.9114.9214.88

[0070] The average value is 14.89 mL, and the standard deviation is 0.028.

Embodiment 3

[0072] Fermentation medium, strains and fermentation system: LB medium, E. coli, 35L LB medium in a 50L tank, and a culture temperature of 37°C.

[0073] The flow rate of the peristaltic pump is 200 mL / min; the inner diameter of the connected silicone tube is 15mm; the inner diameter of the two multi-channel switching valves is 8mm; the connecting pipeline between the multi-port valve and the sample bottle and the waste bottle is an organic plastic hard tube with an inner diameter of 8 mm

[0074] The sampling volume is 50 mL. Set sampling every 2 h for 24 h. The volume of the 12 samples retained is shown in Table 3:

[0075] Table 3 Retained sample results

[0076] frequency123456 Retention sample volume (mL) 49.6549.8149.7249.5949.6149.82 frequency789101112 Retention sample volume (mL) 49.9350.0249.6849.7649.8849.69

[0077] The average value is 49.76 mL, and the standard deviation is 0.133.

[0078] The inner diameter of the silicone tube is directly related to the flow rate ...

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Abstract

The invention relates to a biological reaction process on-line cyclic sampling device and a sampling method. The online cyclic sampling device comprises a circulating pipeline system, a reserved sample pipeline system and a switching system, wherein the circulating pipeline system includes a sampling probe extending into a biological reaction device, a hydraulic power source and a pipeline; the liquid power source drives a liquid in the biological reaction device to enter the pipeline through the sampling probe; the reserved sample pipeline system includes a sample reserving container, an airpressure power source and the pipeline; and the switching system is a multi-channel switching system, and switching control is performed on a backflow channel forming the circulating pipeline system or the reserved sample pipeline system which is connected while the backflow channel is disconnected. Online sampling in a biological reaction process is achieved, sterile sampling is adopted in the sampling method, a sampling amount is less, and sampling interval time is controllable. In addition, a formed dead volume is small, and waste of a liquid sample is greatly reduced.

Description

Technical field [0001] The invention belongs to the field of online sampling devices for a biological reaction process, and particularly relates to an online circulating sampling device and a sampling method for a biological reaction process. Background technique [0002] The biological reaction process is a process in which a biological system performs a biochemical reaction in a biological reaction device. Compared with chemical reactions, biological reactions require milder conditions, slow reaction rate processes, and complex process mechanisms. [0003] The biological reaction process includes three stages: pre-reaction preparation stage, reaction process monitoring and post-reaction treatment. In the process of biological reaction, the implementation of monitoring the state of the culture medium and cell / microbe in the biological reaction is conducive to understanding the state of biological metabolism and provides a very important reference for the optimization of the biolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/10
CPCG01N1/10
Inventor 王立言段保峰姜建星
Owner LUOYANG TMAXTREE BIOTECH CO LTD
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