Helicobacter pylori detection kit

A technology of Helicobacter pylori and detection kit, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of the material, etc., can solve unfavorable screening development, insufficient substrate urea, stable Sexual issues, etc.

Inactive Publication Date: 2020-06-16
南京康容健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the stability of hypochlorite and phenate is not good, and the carrier is agar gel, urea will be decomposed in advance in this system, resulting in insufficient substrate urea during detection, resulting in inability to accurately detect
And it takes 1-3 hours for the test result
[0005] Helicobacter pylori produces urease, which takes a certain amount of time to decompose urea, so the previous rapid urease method takes a lot of time to detect each sample, which is not conducive to large-scale screening.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Dissolve 3 parts of urea, 1 part of nickel chloride, 0.7 part of 1640 culture medium, and 0.5 part of sodium acetate in 100 parts of purified water, and dissolve 0.1 part of phenol red in appropriate ethanol, add them to the above-mentioned purified water, stir to mix Evenly, adjust the pH value to 6.5-6.7 with acetic acid, soak the filter paper in the prepared solution for 12 hours, take it out and dry it, cut it into strips and put it in the kit.

Embodiment 2

[0018] Dissolve 2.5 parts of urea, 0.8 parts of aluminum sulfate, 0.7 parts of DMEM-F12 medium, and 0.5 parts of sodium phosphate in 100 parts of purified water, dissolve 0.1 part of bromothymol blue in appropriate ethanol, and add the above purified water, stir to mix evenly, adjust the pH value to 5.7-5.9 with acetic acid, soak the filter paper in the prepared solution for 12 hours, take it out and dry it, cut it into strips and put it in the kit.

Embodiment 3

[0020] Dissolve 0.8 parts of aluminum trichloride and 0.5 parts of citrate buffer salt in 100 parts of purified water to make reagent A, adjust the pH value to 3.5-3.7 with hydrochloric acid. Fully mix 2.5 parts of urea powder with 0.7 part of DMEM medium powder to prepare granules as reagent B. Dissolve bromocresol green indicator 0.2 in 50 parts of purified water, soak the filter paper in the prepared solution for 12 hours, take it out and dry it, cut it into strips and put it in the kit.

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PUM

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Abstract

The invention provides a kit capable of rapidly detecting helicobacter pylori. The kit adopts a rapid culture method to shorten the detection time, and the kit also uses an ammonia time collecting agent and a buffer pair to improve the detection accuracy and reduce the misjudgment rate of clinical medical personnel. The kit can be used for large-scale clinical screening to prevent helicobacter pylori infection and propagation.

Description

technical field [0001] The application relates to the field of medical equipment, is a kit for detecting Helicobacter pylori, and belongs to the field of medicine. Background technique [0002] Helicobacter pylori lives in the pyloric part of the human stomach and the oral cavity, and is one of the most common bacterial pathogens. Half of the world's population has been infected with Helicobacter pylori, and in some countries almost 90% of the population has been infected with this bacterium. People are usually infected at an early age, reaching 50% under the age of 5 years. This bacterial infection first causes chronic gastritis, leading to gastric ulcer and gastric atrophy, and in severe cases, it develops into gastric cancer. [0003] According to statistics, the incidence of atrophic gastritis and gastric cancer is higher in people who are first infected with Helicobacter pylori at an earlier age, and there is a parallel relationship between Helicobacter pylori infecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 张广明
Owner 南京康容健康科技有限公司
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