The invention discloses a plant culture medium prepared from renewable resources. A preparation method comprises the following steps: 1, cleaning waste plant branches and leaves, drying and smashing; 2, drying animal excrement and smashing; 3, smashing other raw materials separately and sieving through a 80 to 100-mesh sieve; 4, evenly mixing all the following raw materials in parts by weight: 20 to 30 parts of waste plant branch and leaf powder, 10 to 20 parts of animal excrement powder, 20 to 30 parts of charcoal powder, 10 to 20 parts of vermiculite powder, 5 to 10 parts of perlite powder, 5 to 10 parts of medical stone powder, 20 to 30 parts of grass carbon powder, 10 to 20 parts of humus powder, 5 to 10 parts of quartz sand powder, 5 to 10 parts of calcium superphosphate powder and 10 to 15 parts of urea powder. The plant culture medium disclosed by the invention is prepared from the simple renewable resources, and has the advantages of wide raw material source distribution areas, low price, easiness in obtaining and good economic benefit.