Breeding method for rapidly establishing early-flowering resources of Brassica napus

A technology of Brassica napus and spring rape, which is applied in the field of rapidly shortening the growth period of Brassica napus and creating excellent early flowering resources, can solve the problems of heavy workload and low efficiency of breeding methods, so as to avoid phenotype selection, improve selection efficiency, The effect of shortening the breeding cycle

Active Publication Date: 2020-06-26
QINGHAI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: how to provide a breeding method for quickly creating early flowering resources of Brassica oleracea, and solve the problems of heavy workload and low efficiency of existing breeding methods

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  • Breeding method for rapidly establishing early-flowering resources of Brassica napus
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  • Breeding method for rapidly establishing early-flowering resources of Brassica napus

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Embodiment 1

[0070] A method for rapidly creating spring Brassica napus resources, comprising the following steps:

[0071] (1) Using the developed molecular markers closely linked to the early flowering QTL: G1803 and IA7-4 closely linked to the main early flowering QTL cqDTFA7a, S035 and SNP11 closely linked to the main early flowering QTL cqDTFC8 to identify 93 cabbage Type spring rape flowering genotype, construct early flowering genotype library;

[0072] The specific method is as follows: the method for screening a single plant containing the cqDTFA7a site is as follows: using Brassica napus DNA as a template, respectively using the primer pair shown in SEQ ID No.1 and SEQ ID No.2, and the primer pair shown in SEQ ID No.3 Perform PCR amplification with the primer pair shown in SEQ ID No.4, if the amplified products of 330bp and 120bp can be obtained respectively, then the Brassica napus is a single plant containing the cqDTFA7a site;

[0073] The method for screening a single plant ...

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Abstract

The invention discloses a breeding method for rapidly establishing early-flowering resources of Brassica napus. The method comprises the following steps: (1) screening single Brassica napus plants respectively containing a cqDTFA7a site and a cqDTFC8 site by using four molecular markers, (2) hybridizing the single Brassica napus plant containing the cqDTFA7a site and the single Brassica napus plant containing the cqDTFC8 site, (3) carrying out microspore culture on the hybrid F1 generation, carrying out genotype identification on the doubled DH strain by using four molecular marker primer pairs, screening out a DH single plant containing the cqDTFA7a site and the cqDTFC8 site at the same time, and (4) carrying out yield and flowering period identification on the screened DH single plant, and screening out a single plant with excellent yield and flowering period three days earlier than the parent. According to the method, through auxiliary selection of target trait genes through molecular markers, loss of target trait genes in a cross-breeding process is avoided, selection efficiency is improved, breeding period is shortened, and workload is reduced.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and relates to a method for rapidly shortening the growth period of Brassica napus and creating excellent early flowering resources. Background technique [0002] Rapeseed (Brassica campestris L.) is a plant of the genus Brassica in the family Brassicaceae. It is one of the main oil crops in the world, and it is also one of the main oil crops in China. Its planting area accounts for 54% of the total planting area of ​​oil crops in the country. , while China's rapeseed area accounts for 20% of the world's rapeseed area. Brassica napus L. (AACC, n=19) originated in Europe and is one of the three major types of rape. A composite species that evolved from double diploidization after hybridization. Brassica napus has a short growth period and short individuals, and is mainly distributed in high-altitude areas with harsh climate conditions and short frost-free periods; Brassica napus has hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/02A01H1/04A01H6/20
CPCC12Q1/6895A01H1/02A01H1/04A01H6/20C12Q2600/13C12Q2600/156
Inventor 柳海东杜德志
Owner QINGHAI UNIVERSITY
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