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Application of recombinant porcine interleukin 22 in preparation of drugs for improving production performance of pigs

A porcine interleukin, production performance technology, applied in the direction of drug combination, disease, medical formula, etc., can solve the problems that have not yet been reported on the application of IL22, and achieve excellent results and broad application prospects

Pending Publication Date: 2020-06-30
四川华德生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no report on the application of IL22 in improving pig production performance

Method used

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  • Application of recombinant porcine interleukin 22 in preparation of drugs for improving production performance of pigs
  • Application of recombinant porcine interleukin 22 in preparation of drugs for improving production performance of pigs
  • Application of recombinant porcine interleukin 22 in preparation of drugs for improving production performance of pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Synthesis of embodiment 1 recombinant porcine interleukin 22 gene (SsIL22)

[0025] 1. Gene sequence analysis

[0026] The known porcine interleukin 22 gene was retrieved from the NCBI database, and the signal peptide part of the interleukin 22 was removed at the same time to obtain the following sequence (SEQ ID NO.1):

[0027]

[0028] The sequence is 462bp in total, encoding a protein with 153 amino acid residues (SEQ ID NO.2):

[0029]

[0030]

[0031] 2. DNA sequence synthesis of porcine interleukin 22

[0032] The porcine interleukin 22 gene sequence was analyzed, and a new IL22 gene was designed and synthesized according to the codon preference of Pichia pastoris, named IL22-n. The sequence (SEQ ID NO.3) is as follows:

[0033]

Embodiment 2

[0034] Example 2 Expression and Analysis of Porcine Interleukin 22 Gene (SsIL22) in Yeast (1) Method

[0035] 1. Construction of pGAP-IL22-n vector

[0036] The whole gene IL22-n was cloned into the Pichia pastoris constitutive expression vector pGAPZ-alpha vector through the EcoRI / NotI site to obtain the recombinant vector pGAP-IL22-n. The DNA recombination operation is mainly carried out according to the "Molecular Cloning Experiment Manual".

[0037] 2. Electroporation transformation and screening of yeast

[0038] The recombinant vector pGAP-IL22-n was digested with Bln I to linearize it, and transformed into Pichia pastoris by electroporation. For electrotransformation and screening methods, refer to the Invitrogen Company Operation Manual. Colonies were taken for PCR and gel electrophoresis to detect the transformation of IL22. Pick positive transformants (such as figure 1 shown).

[0039] 3. Expression of Recombinant Yeast

[0040] Recombinant yeast was cultured ...

experiment example

[0055] Experimental example Animal experiment

[0056] By adding IL22 fermentation broth with IL22 content of 10 μg / mL and pGAP carrier-transformed strain GS 115-pGAP without IL22 gene in the diet of nursery piglets as controls, the animal use effect of porcine interleukin 22 was verified.

[0057] 1 Test materials and methods

[0058] 1.1 Test animals

[0059] This experiment completely followed the random selection of piglets in the nursery stage with different body weights and good eye health and development.

[0060] 1.2 Test time and place

[0061] The initial age of the test was 25 days, the end age was 57 days, and the test period was 32 days. The experiment was conducted at Zhongjiang Dedicated Agriculture and Animal Husbandry Company from September 6th to October 8th, 2018.

[0062] 1.3 Test animals

[0063]Select 180 pigs with similar age, weight and health level, and divide them into 3 groups on average, namely blank control group, IL22 group and IL22 control g...

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Abstract

The invention provides application of IL22 in the preparation of drugs for improving the production performance of pigs. A DNA sequence of a recombinant porcine interleukin 22 is shown as SEQ ID NO.3.The drugs can be a fermentation broth obtained by eukaryotic fermentation of IL22, or an oral preparation prepared by IL22 combined with a pharmaceutically acceptable auxiliary material or carrier. The food intake of pigs can be significantly increased, the diarrhea rate of pigs can be reduced, so that the feed conversion rate is reduced, and the growth performance of pigs is improved.

Description

technical field [0001] The invention relates to the field of animal immunity, in particular to the application of recombinant porcine interleukin 22 in the preparation of medicines for improving pig production performance. Background technique [0002] Pig farming is a traditional industry in my country. Since the reform and opening up, my country’s pig industry has been strongly driven by demand caused by sustained and rapid economic growth, rising income levels of urban and rural residents, and continuously upgrading food consumption structures. On the other hand, the pig industry has evolved. It has become an important source of income for rural residents and an important part of the vegetable basket project for urban residents and has been strongly promoted by the government, which has maintained a relatively rapid growth momentum in my country's hog production for a long time. [0003] In order to maintain the health status of live pigs and make them have better growth p...

Claims

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Application Information

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IPC IPC(8): A61K38/20A61P15/08
CPCA61K38/20A61P15/08
Inventor 李钢邹辉琴温静康健王定越
Owner 四川华德生物工程有限公司
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