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Simultaneous high-flux automatic detector for various viruses

A high-throughput, detector technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of insufficient sensitivity and accuracy, achieve the effects of shortening observation time, high efficiency, and reducing the risk of biological infection

Pending Publication Date: 2020-07-17
SHANGHAI INST OF TECHNICAL PHYSICS - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason why there are so many false negatives, in the final analysis, is that the sensitivity and accuracy are not high enough

Method used

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  • Simultaneous high-flux automatic detector for various viruses
  • Simultaneous high-flux automatic detector for various viruses
  • Simultaneous high-flux automatic detector for various viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: High-throughput detection of new coronavirus

[0034] Simultaneous high-throughput automatic detector for multiple types of viruses includes an inactivation temperature controller 1, an automatic transfer device 2, an amplification temperature controller 3, an automatic feeding device 4, an excitation light source array 5, a light source filter 6, and a fluorescence filter 7. Lens 8, array photodetector 9, data processing display and transmission module 10, USB power supply interface 11. Wherein the central wavelength of the LED excitation light source array is 480nm, and the transmission spectrum of the light source filter 6 is as follows: figure 2 As shown, the transmission spectrum of the fluorescence filter 7 is as image 3 As shown, the array photodetector 9 is CMOS.

[0035] The specific operation steps are as follows:

[0036] First, download and install the "Mobile Nucleic Acid Detection" mobile app, then power on the detector, click the device co...

Embodiment 2

[0037] Example 2: High-throughput simultaneous detection of multiple viruses

[0038] Simultaneous high-throughput automatic detector for multiple types of viruses includes an inactivation temperature controller 1, an automatic transfer device 2, an amplification temperature controller 3, an automatic feeding device 4, an excitation light source array 5, a light source filter 6, and a fluorescence filter 7. Lens 8, array photodetector 9, data processing display and transmission module 10, USB power supply interface 11. Wherein the central wavelength of the LED excitation light source array is 480nm, and the transmission spectrum of the light source filter 6 is as follows: figure 2 As shown, the transmission spectrum of the fluorescence filter 7 is as image 3 As shown, the array photodetector 9 is a CCD.

[0039] The specific operation steps are as follows:

[0040] First, download and install the "Mobile Nucleic Acid Detection" mobile app, then power on the detector, clic...

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Abstract

The invention discloses a simultaneous high-flux automatic detector for various viruses. The detector is composed of a temperature controller, an automatic transfer device, an automatic feeding device, an excitation light source array, a light source optical filter, a fluorescence optical filter, an array photoelectric detector, a data processing display and transmission module, a USB power supplyinterface and the like. The detector integrates inactivation, nucleic acid extraction, amplification and high-throughput detection, and can be used for the high-throughput simultaneous detection of various viruses such as novel coronavirus, influenza, African swine fever, avian influenza, Ebola and the like. A to-be-detected sample only needs to be put into the instrument, the sample can be automatically detected at the same time through remote control of a mobile phone APP, and the data is automatically sent to a background for big data cloud processing and analysis. The detector is simple and convenient to operate, intelligent, safe, high in speed and high in sensitivity and can simultaneously detect massive samples of various viruses, and the purpose of rapid screening is achieved.

Description

technical field [0001] The invention relates to the field of medical testing instruments, in particular to an instrument capable of realizing simultaneous high-throughput detection of multiple viruses. Background technique [0002] At present, the frontline of the anti-epidemic mainly uses RT_PCR detection and CT examination to carry out the examination of new crown patients. RT_PCR detection is the gold standard, but it takes a long time and requires high requirements for professional instruments and personnel, thus creating a "barrier lake" for early virus detection. [0003] In addition, many companies have developed new crown-specific antibody detection products, which are undergoing clinical testing and verification. Specific antibody detection has high detection sensitivity and specificity, is suitable for large-scale screening, and is an important supplement to nucleic acid detection, but the disadvantage is that the early diagnosis of infected people has certain lim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 陆卫郑伟波王少伟丁昆张涛
Owner SHANGHAI INST OF TECHNICAL PHYSICS - CHINESE ACAD OF SCI
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