Application of ginsenoside to wound repair
A technology of ginsenosides and skin wounds, applied in the application field of ginsenosides in repairing wounds
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[0014] 1. Materials and methods
[0015] 1.1 Materials Ginsenoside Ro, Shanghai Ronghe Biotechnology Co., Ltd.; MTT, Sigma; RPMI-1640 culture medium, Hyclone; microplate reader, BioTek, USA.
[0016] 1.2 Cell culture The cell line HaCaT human keratinocytes was preserved by the Laboratory of Changhai Hospital of Traditional Chinese Medicine Affiliated to Second Military Medical University. Use 1640 culture medium plus 10% fetal bovine serum (FBS), 100 μg / mL penicillin, 100 μg / mL streptomycin, at 37 ° C, 95% air and 5% CO 2 Cultured under the condition of , the logarithmic growth phase cells were taken for experiments.
[0017] 1.2 Experimental method
[0018] 1.2.1 Detection of cell viability by MTT method Use trypsin-EDTA to digest HaCaT into single cells, add 4000 cells / well to a 96-well plate, 100 μL per well. After the cells adhered to the wall, the old culture medium was discarded, and different concentrations of ginsenoside Ro culture medium (80 μM, 40 μM, 20 μM, 10 μM...
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