Application of ginsenoside to wound repair

A technology of ginsenosides and skin wounds, applied in the application field of ginsenosides in repairing wounds

Pending Publication Date: 2020-07-28
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no report about the application of ginsenosides in repairing wounds.

Method used

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  • Application of ginsenoside to wound repair
  • Application of ginsenoside to wound repair
  • Application of ginsenoside to wound repair

Examples

Experimental program
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Effect test

Embodiment 1

[0014] 1. Materials and methods

[0015] 1.1 Materials Ginsenoside Ro, Shanghai Ronghe Biotechnology Co., Ltd.; MTT, Sigma; RPMI-1640 culture medium, Hyclone; microplate reader, BioTek, USA.

[0016] 1.2 Cell culture The cell line HaCaT human keratinocytes was preserved by the Laboratory of Changhai Hospital of Traditional Chinese Medicine Affiliated to Second Military Medical University. Use 1640 culture medium plus 10% fetal bovine serum (FBS), 100 μg / mL penicillin, 100 μg / mL streptomycin, at 37 ° C, 95% air and 5% CO 2 Cultured under the condition of , the logarithmic growth phase cells were taken for experiments.

[0017] 1.2 Experimental method

[0018] 1.2.1 Detection of cell viability by MTT method Use trypsin-EDTA to digest HaCaT into single cells, add 4000 cells / well to a 96-well plate, 100 μL per well. After the cells adhered to the wall, the old culture medium was discarded, and different concentrations of ginsenoside Ro culture medium (80 μM, 40 μM, 20 μM, 10 μM...

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Abstract

The invention relates to an application of ginsenoside Ro to preparation of a skin wound repair drug. The skin wound is caused by keratinocyte injury or caused by keratinocyte damage. The applicationhas advantages as follows: a HaCaT human keratinocyte experiment indicates that Ro has an obvious proliferative effect on HaCaT cells, and with the increase of time, the proliferation rate of the HaCaT cells tends to be reduced; and when concentration is increased gradually, the proliferation rate of the HaCaT cells is also increased. After ginsenoside Ro treatment is carried out for 16 h, it canbe observed under a light microscope that when the cells of a Ro treatment group are compared with the cells of a control group, the migration distances have no significant difference. After Ro treatment, the HaCaT G0 / G1 ratio is reduced, the S-phase cell ratio changes a little, and the G2 / M cell ratio is slightly increased, however, no obvious trend of time or dosage dependence is produced.

Description

technical field [0001] The invention relates to the technical field of medicines, and in particular relates to the application of ginsenosides in repairing wounds. Background technique [0002] The skin is an important organ of the human body, composed of epidermis, dermis and subcutaneous tissue. The basic functions of the skin have five aspects: protection function, temperature regulation function, sensory function, perspiration function and absorption function. At the same time, it has important physical, chemical and biological barrier functions. Wound is the damage of normal skin (tissue) caused by external injury factors such as surgery, external force, heat, electric current, chemical substances, low temperature, and internal factors such as local blood supply disturbance; often accompanied by skin integrity The destruction of the skin and the loss of a certain amount of normal tissue. At the same time, the normal function of the skin is damaged, also known as wound ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P17/02
CPCA61K31/704A61P17/02
Inventor 杨延龙陈婷如苏永华殷子斐
Owner SHANGHAI CHANGHAI HOSPITAL
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