Application of neurotrophic factor 3 in preparation of medicine for treating male hypogonadism

A technology of neurotrophic factors and hypogonadism, which is applied in drug combinations, diseases, and pharmaceutical formulas, etc., can solve problems such as research on male hypogonadism that has not been found in neurotrophic factors 3, and achieve the cure of male hypogonadism, increase Testosterone levels, the effect of increasing the number

Pending Publication Date: 2020-08-11
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No research has been found on neurotrophic factor 3 in the treatment of male hypogonadism

Method used

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  • Application of neurotrophic factor 3 in preparation of medicine for treating male hypogonadism
  • Application of neurotrophic factor 3 in preparation of medicine for treating male hypogonadism
  • Application of neurotrophic factor 3 in preparation of medicine for treating male hypogonadism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. The effect of neurotrophic factor 3 on the number of Leydig cells.

[0039] Take 18 male Sprague-Dawley rats (90 days old). Intraperitoneal injection of dimethylethanesulfonic acid (EDS, dose 75mg / kg body weight) kills Leydig cells, but has no effect on Leydig stem cells, and then divided into 3 groups (6 rats in each group). Grouping is as follows: 1) blank (normal saline); 2) neurotrophic factor 3 (10ng / testis / d); 3) neurotrophic factor 3 (100ng / testis / d). From the 14th day after EDS injection, the ingredients and doses specified in the above groups were injected into the testes every day to the 28th day after EDS injection (this stage is the regeneration and differentiation of Leydig stem cells into new Leydig cells). After death by carbon dioxide, the testes were taken out, fixed in Bouin's solution for 48 hours, and then embedded in dehydrated paraffin to make a tissue chip. Immunohistochemical methods were used to stain and count the Leydig cell-specific prote...

Embodiment 2

[0058] 1. Effect of neurotrophic factor 3 on the number of testicular mesenchymal stem cells and the content of testosterone in testis

[0059] Take male Sprague-Dawley rats (90 days old), inject 75mg / kg EDS intraperitoneally 7 days before the experiment, kill them with carbon dioxide, take out the testes, place them in ice-cold phosphate buffer, cut off the capsule, peel off the blood vessels and seminiferous tubules, The seminiferous tubules were separated into single ones, and the seminiferous tubules were evenly divided into 12-well plates, and DMEM / 12 medium (sigma company) containing different concentrations of neurotrophic factor 3 was added to treat for 7 days, and the specific grouping was 0ng / ml (control group), 10ng / ml and 100ng / ml neurotrophic factor 3. After 7 days, a part of seminiferous tubules was detected with EdU (Life Technologies company) proliferation kit (results in figure 2 ), the other part of the seminiferous tubules was removed from the neurotrophic...

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Abstract

The invention provides application of a neurotrophic factor 3 in preparation of a medicine for treating male hypogonadism, and belongs to the technical field of sexual function medicines. The neurotrophic factor 3 has the effects of stimulating the proliferation of organism testicular mesenchymal stem cells, increasing the number of testicular mesenchymal cells and improving or treating low testosterone in serum and / or testis, and is used as an active ingredient to achieve the effect of treating male hypogonadism syndrome.

Description

technical field [0001] The invention belongs to the technical field of sexual function drugs, and in particular relates to the application of neurotrophic factor 3 in the preparation of drugs for treating male hypogonadism. Background technique [0002] Male hypogonadism is a disease of hypogonadism caused by the lack or reduction of androgen or the inability to exert its function. The cytoplasm of Leydig cells is rich in mitochondria and smooth endoplasmic reticulum, and its main function is to synthesize testosterone, which is the main source of androgen in males. Leydig cells are divided into four distinct stages during differentiation and development: Stem Leydig cells, Progenitor Leydig cells, Immature Leydig cells, and adult mesenchymal cells. Cell (Adult Leydig cell). In these developmental processes, abnormal proliferation and differentiation, reduced quantity, and decreased function of hormone synthesis and secretion will also lead to androgen deficiency in the bo...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61P15/08
CPCA61K38/185A61P15/08Y02A50/30
Inventor 王义炎李晓珩葛仁山李慧涛朱琦琦余弋戈
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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