Intelligent culture and remote observation culture device and method

Abstract

The invention provides an intelligent culture and remote observation culture device and method. The intelligent culture and remote observation culture device comprises a culture box, a manipulator, shelves, a culture dish storing and taking platform, an observation platform, a microscope, an image acquisition device, a detection device, an environment adjustment device, a control unit and an intelligent terminal; the culture dish storing and taking platform, the observing platform and the multiple shelves are arranged in the culture box in an arc shape, and mechanically installed at the circlecenter of the arc; a plurality of rows of partition boards are arranged on the shelves; the microscope is inversely mounted below the observation platform, and movement of a culture dish and focusingof the microscope are performed through a movable focusing mechanism; and the control unit is connected with the manipulator, the image acquisition device, the detection device, the environment adjusting device and the intelligent terminal. According to the intelligent culture and remote observation culture device and method, the cell culture process is not interrupted, meanwhile, cells can be observed in time, a culture target is observed by using the intelligent terminal, the observation efficiency and accuracy are improved, the artificial influence on the culture result is reduced, and theinfluence of electromechanical equipment on the cell culture environment is reduced.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to an intelligent culture and remote observation culture device and method. Background technique [0002] At present, cell culture equipment is a simple function realization equipment with a low degree of automation and intelligence. It is basically a separation equipment, and many links require human participation or complete manual implementation. The control of the temperature, humidity, and gas concentration in the incubator and the remote observation of the cell culture status cannot be realized at the same time. During the cultivation process, the operator should open the hatch of the incubator from time to time, take out the culture dish, transfer the culture target, and then observe , and then make artificial analysis, then put it back into the original incubator, and then repeat the previous actions until the cell growth reaches the target. This operation ...

AI Technical Summary

Problems solved by technology

This operation process is cumbersome, reduces the work efficiency of the operator, and will greatly destroy the ideal growth environment of the cells, not only destroying the cell growth environment that the operator wants to see, but also destroying the growth environment of other cells in the incubator

After the cells are taken out, they are transported to the microscope observation place in an environment that is not suitable for cell growth. It is necessary to go through complex processes such as focusing, observing, moving, observing, and comparing to complete the operation process. However, this process takes a long time and the environment Just a normal environment with unpredictable effects on cell growth

At the same time, different concepts of time and space of different operators will affect cell growth to varying degrees, thereby destroying the consistency of cell culture and affecting the culture results of the culture target from the ideal state.

[0003] At present, most of the cell culture equipment is box-type structure, the storage quantity of stem cell culture dishes is limited, the culture dishes are stacked randomly on each floor, there is no clear position, the size of the culture dish cannot be identified, and it cannot adapt to the automation of cell culture

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