Novel adherent cell culture device

A technology for adherent cells and culture devices, which can be used in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc., and can solve problems such as troublesome operation

Pending Publication Date: 2020-09-01
南京九川科学技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current new adherent cell culture device is only used as a container for cell culture. When the growth status of the cells is needed, the culture container in the prior art needs to be taken out of the incubator, and then placed under the microscope for observation. Then put i

Method used

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  • Novel adherent cell culture device
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] The present invention includes a novel adherent cell culture device, such as Figure 1-3 shown, including:

[0080] PCB circuit board 5;

[0081] The image acquisition device 20 is arranged on the upper surface of the PCB circuit board 5, and the image acquisition device 20 is connected with a controller 9;

[0082] The cell culture container 1 provides a container for adherent cells. The cell culture container 1 is arranged above the image acquisition device 20, and the bottom of the side wall of the cell culture container 1 is attached to the upper surface of the image acquisition device 20. The cell culture container 1 The bottom is provided with an opening 3 exposing the image acquisition device;

[0083] The image collection device 20 collects images of the adherent cells through the opening 3, and outputs the collected images to the controller 9;

[0084] The controller 9 is used to process the image to obtain the growth data of the adherent cells, and transmit...

Embodiment 2

[0172] The present invention includes a novel adherent cell culture device, such as Figure 7-10 shown, including:

[0173] PCB circuit board 5;

[0174] The image acquisition device 20 is arranged on the upper surface of the PCB circuit board 5, and the image acquisition device 20 is connected with a controller 9;

[0175] The cell culture container 1 provides a container for the adherent cells, the cell culture container 1 is placed above the image acquisition device 20, and the bottom of the cell culture container 1 is provided with an opening 3 for exposing the image acquisition device 20;

[0176] The image collection device 20 collects images of the adherent cells through the opening 3, and outputs the collected images to the controller 9;

[0177] The controller 9 transmits the processed growth data of the adherent cells to an external display terminal.

[0178] In the above-mentioned embodiment, the cell culture container 1 is directly arranged above the image acqui...

Embodiment 3

[0256] A novel adherent cell culture device, such as Figure 8 As shown, it includes a circular culture container 1 for culturing adherent cells and an imaging chip 2 for image acquisition. The top surface of the culture container 1 is open, and an imaging hole 3 is opened on the bottom surface. A culture tank 8 is included in the container 1, the central axis of the imaging hole 3 is the same as that of the culture tank 8, the shapes of the imaging hole 3 and the culture tank 8 are both circular, and the imaging chip 2 is packaged in Inside the package 4, the back side of the package 4 is provided with electrodes packaged with the imaging chip 2 for input and output between the imaging chip 2 and the external control system, and the upper surface of the package 4 is connected to the The lower surface of the culture container 1 is bonded without gaps with an adhesive.

[0257] The casing 4 with the imaging chip 2 packaged in the PLCC method is welded to the PCB circuit board ...

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Abstract

The invention provides a novel adherent cell culture device. The novel adherent cell culture device comprises a PCB; an image acquisition device arranged on the upper surface of the PCB, wherein the image acquisition device is connected with the controller; a cell culture container used for providing a container for adherent cells, wherein the cell culture container is arranged on the image acquisition device, the bottom of the side wall of the cell culture container is attached to the upper surface of the image acquisition device, and an opening for exposing the image acquisition device is formed in the bottom of the cell culture container, the image acquisition device being used for acquiring images of the adherent cells through the opening and outputting the acquired images to a controller; and the controller used for processing the images to obtain growth data of the adherent cells and transmitting the growth data to an external display terminal. The device has the advantages thatreal-time monitoring of adherent cells in the cell culture vessel is achieved, so that the monitoring precision of the adherent cells is improved, meanwhile, the real-time monitoring space is saved, and then the monitoring cost is reduced.

Description

technical field [0001] The invention relates to the technical field of biological cell culture, in particular to a novel adherent cell culture device. Background technique [0002] Cell culture is an essential process in bioengineering technology, which can transform a single cell into a simple single cell or rarely differentiated multi-cell through mass culture. A large number of cells or their metabolites can be obtained by cell culture. Since most biological products are obtained from cells, cell culture technology is the core and basic technology in biotechnology. Cell culture work has been widely used in various fields such as biology, medicine, and new drug research and development, and it plays a vital role in the entire life science research. [0003] However, the current new adherent cell culture device is only used as a container for cell culture. When the growth status of the cells is needed, the culture container in the prior art needs to be taken out of the in...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/34C12M1/36
CPCC12M41/36C12M41/48
Inventor 杨程卢大骅
Owner 南京九川科学技术有限公司
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