The invention discloses an austenite alloy EBSD sample preparation method. The preparation method comprises austenite alloy sample preparation, grinding, polishing, fine polishing, two-step ultrasonictreatment and drying steps, wherein for the fine polishing step, in the fine polishing process, silica suspension liquid with particle size of 20-50nm is used, the rotation speed of a metallographical polishing machine is smaller than or equal to 100 turn/min, and fine polishing time is larger than or equal to 10 min; and for the two-step ultrasonic treatment step, the first ultrasonic treatmentstep is characterized by placing the samples in a 10%NaOH aqueous solution, which is 10-45 DEG C, for ultrasonic cleaning, the cleaning time being larger than or equal to 3 min. Compared with the national standard GBT19501, the method does not need the electropolishing process and does not need to purchase special equipment, thereby reducing preparation steps, making preparation simple and convenient and reducing cost; and the problems that an existing electropolishing method needs special equipment, is high in sample preparation cost and is long in polishing process exploration time, and electrolyte pollutes the environment and the like are overcome effectively.