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Method for screening tetracycline-resistant bacteria from household water purifier filter element

A tetracycline and water purifier technology, which is applied in the field of microbial screening, can solve problems such as inability to judge the biological risk of water purification equipment, and achieve the effects of ideal screening results, simple and easy operation, and improved efficiency.

Pending Publication Date: 2020-10-27
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the existence of antibiotic-resistant bacteria in water purifier filters, and more research is on the screening and cultivation of resistant bacteria in environmental water bodies, and it is impossible to judge whether there is a biological risk in the most common water purification equipment in residents' homes

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0038] A method for screening tetracycline-resistant bacteria from household water purifier filter cartridges, comprising the following steps:

[0039] 1) Under sterile conditions, prepare tetracycline storage solution, several parts of liquid enrichment medium and several parts of solid-state selective medium;

[0040] Preparation of tetracycline storage solution: Tetracycline storage solution was prepared with absolute ethanol, sterilized by filtration through a 0.22 μm filter membrane, 1 mL / portion, and stored in a -20°C refrigerator in the dark, and the storage time did not exceed 30 days.

[0041] Preparation of liquid enrichment medium: Accurately weigh 10g of peptone, 5g of yeast powder, and 10g of NaCl, add ultrapure water to a volume of 1000mL, dissolve and keep stirring, autoclave at 120°C for 20min before use, and put the sterilized medium into Put it in a water bath at 45°C ± 1°C to cool it down for later use.

[0042]Preparation of solid selective medium: Accurat...

Embodiment 2

[0054] A method for screening tetracycline-resistant bacteria from household water purifier filter cartridges, comprising the following steps:

[0055] 1) Under sterile conditions, prepare aureomycin stock solution, several parts of enrichment culture solution and several parts of solid-state selection medium;

[0056] Preparation of chlortetracycline stock solution: Prepare chlortetracycline stock solution with absolute ethanol, filter and sterilize through a 0.22 μm filter membrane, aliquot 1 mL / portion, and store in a -20°C refrigerator in the dark for no more than 30 days.

[0057] Preparation of liquid enrichment medium: Accurately weigh 10g of peptone, 5g of yeast powder, and 10g of NaCl, add ultrapure water to a volume of 1000mL, dissolve and keep stirring, autoclave at 120°C for 20min before use, and put the sterilized medium into Put it in a water bath at 45°C ± 1°C to cool it down for later use.

[0058] Preparation of solid selective medium: Accurately weigh 10g of...

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Abstract

The invention belongs to the technical field of microbiological screening, and particularly relates to a method for screening tetracycline-resistant bacteria from a household water purifier filter element. The method comprises the following steps of: preparing a tetracycline antibiotic storage solution, a plurality of liquid enrichment media and a plurality of solid selective media; preparing a plurality of liquid enrichment media and solid selective media containing tetracycline antibiotics of different concentrations; and separating and purifying the tetracycline-resistant bacteria in the household water purifier filter element. Compared with the prior art, the method is simple and easy to operate, is safe and environmentally friendly and can effectively screen goal bacteria.

Description

technical field [0001] The invention belongs to the technical field of microbial screening, and in particular relates to a method for screening tetracycline-resistant bacteria from household water purifier filter elements. Background technique [0002] In recent years, the use of a large number of irrational antibiotics has led to an increasing number and types of resistant bacteria in groundwater, soil, and air, and has gradually evolved from single-drug resistance to multi-drug resistance, even in drinking water. Both can detect the presence of resistant bacteria. Antibiotics in tap water may induce the enrichment of antibiotic-resistant microorganisms, and resistance genes can enable microorganisms to acquire multiple antibiotic resistances through horizontal transfer. The emergence of "super bacteria" has caused irreversible disasters to human health. [0003] With the rapid improvement of the social and economic level, consumers are paying more and more attention to th...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/20C12R1/42C12R1/19
CPCC12N1/36C12N1/20
Inventor 翟洪艳程胜梓
Owner TIANJIN UNIV
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