A kind of culture medium for preserving test-tube plantlets of hosta mosaic and its application

A technology of hosta mosaic and test-tube seedlings, which is applied in the field of plant tissue culture, can solve the problems of short storage period, degradation and variation of test-tube seedlings, and achieve the effects of low cost, simple operation, and reduced storage costs

Active Publication Date: 2021-11-16
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The culture medium of the present invention aims at the short storage period in the prior art, the need for frequent subculture renewal, and multiple subcultures can easily lead to the degradation or variation of test-tube plantlets, which can make up for the deficiencies in the prior art. Hosta mosaic test-tube plantlet medium

Method used

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  • A kind of culture medium for preserving test-tube plantlets of hosta mosaic and its application
  • A kind of culture medium for preserving test-tube plantlets of hosta mosaic and its application
  • A kind of culture medium for preserving test-tube plantlets of hosta mosaic and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 method for preserving test-tube seedlings of hosta mosaic

[0029] Experimental materials: The test-tube seedlings of Hosta mosaic came from the test-tube seedling bank of the National Crop Germplasm Bank.

[0030] The specific steps of the experimental method are as follows:

[0031] (1) The growth method after the test-tube plantlet is multiplied is as follows: the aseptic test-tube plantlet of the hosta mosaic that is proliferated and multiplied is under the conditions of a culture temperature of 20-25°C, a light intensity of 2000-3000lx, and a light time of 12-14h / d. , into MS+3% sucrose+0.7% agar (pH5.8) culture medium for 30-45 days

[0032](2) The delayed growth preservation method is as follows: Select the aseptic test-tube plantlets of Hosta mosaic with 4 leaves and 1 heart (5 ± 0.5 cm in height) that are robust and consistent in growth in step (1) and inserted into the delayed growth preservation medium MS+ 50g / L sucrose + 20g / L mannitol + 1.0mg...

Embodiment 2

[0035] The survival rate of embodiment 2 hosta test-tube plantlets preserved after 6 months

[0036] Take the aseptic seedlings of Hosta mosaic that grow vigorously and grow uniformly (4 leaves and 1 heart, height is 5 ± 0.5cm) after being cultivated on MS+3% sucrose+0.7% agar (pH5.8) medium for 30 to 45 days. The materials were respectively inserted into the medium of 11 kinds of preservation schemes, and the culture conditions were the same as in Example 1, and the influence of the culture medium of each preservation scheme on the survival rate was studied. The results are shown in Table 1.

[0037] Table 1 The survival rate of hosta mosaic in each preservation scheme for 6 months

[0038]

[0039]

[0040] As can be seen from Table 1, although the survival rate of the control group was still 100% when preserved for 3 months, the test-tube plantlets had been topped with bottle caps and there was little left in the culture medium, so it was necessary to subculture. Re...

Embodiment 3

[0041] Example 3 Changes in Plant Height in Delayed Growth and Preservation of Hosta Mosaic Test-tube Plantlets

[0042] (1) grow robustly and grow uniformly (4 leaves and 1 heart, height is 5 ± 0.5cm) after cultivating on MS+3% sucrose+0.7% agar (pH5.8) medium for 30 to 45 days. Bacteria were used as materials, which were respectively inserted into the control medium (MS+3% sucrose+0.7% agar, pH5.8) and the medium of the delayed growth preservation scheme (MS+50g / L sucrose+20g / L mannitol+1.0mg / L abscisic acid (ABA)+20mg / L chlormequat (CCC)+0.7% agar, pH5.8) is the medium in the A scheme of embodiment 2, and culture condition is the same as embodiment 1, researches in embodiment 2 Effect of protocol A medium on plant height of surviving plants. The results are shown in Table 2, figure 1 , figure 2 shown.

[0043] Table 2 Plant height changes of Hosta mosaic during preservation

[0044]

[0045] It can be seen from Table 2 that with the prolongation of storage time, t...

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Abstract

The invention discloses a culture medium for preserving test-tube seedlings of hosta mosaic and an application thereof, belonging to the technical field of plant tissue culture. The medium for preserving test-tube plantlets of hosta mosaicus of the present invention is an MS medium containing sucrose, mannitol, abscisic acid, chlormequat and agar. The culture medium of the present invention is used for preserving test-tube seedlings of Hosta mosaic. The test-tube seedlings of Hosta mosaic can be preserved for 6 months. After preservation, the material survival rate is 100%, and the phenotype of the plant is stable after recovery and culture, without degeneration and variation. The culture medium of the invention has low cost, overcomes the shortcomings of short storage time and easy variation of phenotype after storage in the existing storage medium, can be applied to the storage of hosta germplasm resources, and has great application value.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a medium for preserving test-tube seedlings of hosta mosaicus and its application. Background technique [0002] Hosta plantaginea is a perennial herbaceous plant of the Liliaceae Hosta genus. It is a variant of the famous foliage plant Hosta. It has strong complementarity to light-absorbing flowers and is an excellent variety for landscaping. The plants of Hosta mosaic are dense, the leaves are ovate to heart-shaped, with yellow streaks, and there are many cultivars. The leaf shape, leaf color and plant type are very different. The green ones are verdant, and the blue ones are faint. Elegant, brilliantly colored foliage in magical gradations of color. In the upsurge of cultivating new varieties of the genus Hosta in the world, Hosta mosaic stands out among the hosta varieties because of its unique leaf color. Therefore, Hosta mosaic has become a wild f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
CPCA01H4/001A01H4/008A01N3/00
Inventor 陈晓玲张金梅李洋卢新雄辛霞尹广鹍何娟娟刘运霞黄雪琦
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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