Epithelial cell culture medium
A breast epithelial cell and culture medium technology, applied in the field of epithelial cell culture medium, can solve the problems of prone to apoptosis, long experiment cycle, and inconvenient research
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[0016] A kind of cell culture fluid, the concentration ratio of each component is as follows:
[0017]
[0018] Weigh each component with the above weight, put it in an appropriate container, add 950mL ultrapure water and stir to dissolve for 10-15 minutes, use 0.1mol / L sodium bicarbonate solution to adjust the pH to 6.8-7.2, add 50mL fetal bovine serum, Make the fetal calf serum account for 5% of the total volume, and finally use a 0.45 micron microporous membrane to filter, collect the filtrate, and store it for use.
[0019] Effects of different culture media on the proliferation rate of dairy cow mammary gland epithelial cells in vitro:
[0020] Inoculate dairy cow mammary gland epithelial cells (100 μL / well) in a 96-well plate, the number of cells is 1000 / well, place the culture plate in an incubator for pre-culture (37°C, 5% CO2) for 12 hours, and replace the test cells in each group For the culture solution, continue to cultivate in the incubator for 24h, 48h, and 7...
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