Epithelial cell culture solution

A technology of epithelial cells and culture fluids, applied in the direction of cell culture active agents, epidermal cells/skin cells, animal cells, etc., which can solve the problems of research inconvenience, long experiment period, poor resistance to environmental changes, etc.

Active Publication Date: 2020-10-30
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the commonly used cell culture medium only adds fetal bovine serum and antibiotics on the basis of DMEM basic culture medium. Some cell lines have poor reproductive ability, poor resistance to environmental changes, and are prone to apoptosis or even necrosis.
It leads to a long experiment cycle and low experiment efficiency, which brings inconvenience to the research based on cell culture in vitro

Method used

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  • Epithelial cell culture solution
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Experimental program
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specific Embodiment 1

[0016] A kind of cell culture fluid, the concentration ratio of each component is as follows:

[0017]

[0018] Weigh each component with the above weight, put it in an appropriate container, add 950mL ultrapure water and stir to dissolve for 10-15 minutes, use 0.1mol / L sodium bicarbonate solution to adjust the pH to 6.8-7.2, add 50mL fetal bovine serum, Make the fetal calf serum account for 5% of the total volume, and finally use a 0.45 micron microporous membrane to filter, collect the filtrate, and store it for use.

[0019] Effects of different culture media on the proliferation rate of dairy cow mammary gland epithelial cells in vitro:

[0020] Inoculate dairy cow mammary gland epithelial cells (100 μL / well) in a 96-well plate, the number of cells is 1000 / well, place the culture plate in an incubator for pre-culture (37°C, 5% CO2) for 12 hours, and replace the test cells in each group For the culture solution, continue to cultivate in the incubator for 24h, 48h, and 7...

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Abstract

The invention discloses an epithelial cell culture solution which comprises the following components: 13.4 g / L of DMEM, 5% of fetal calf serum, 10-30mg / L of baicalin, 1-4.5 mg / L of resveratrol, 300-500mg / L of glutathione, 60mg / mL of penicillin and 100mg / L of streptomycin. According to the invention, external stimulation is reduced in the culture process of cells, the proliferation speed of the cells is increased, and the cells meeting the required quantity can be obtained more quickly.

Description

technical field [0001] The invention relates to an epithelial cell culture fluid. Background technique [0002] Cell culture refers to a method of artificially simulating the internal environment in vitro to enable it to survive, grow, reproduce and maintain its main structure and function. Cell culture medium is the material basis for providing nutrition and ensuring the normal growth of cells. The in vitro culture of cells is widely used in the fields of biotechnology and medicine, and is of great significance for the research of human and animal diseases, the development of drugs and even the development of new foods. [0003] At present, the commonly used cell culture medium only adds fetal bovine serum and antibiotics on the basis of DMEM basal culture medium. Some cell lines have poor reproductive ability, poor resistance to environmental changes, and are prone to apoptosis or even necrosis. This results in a long experiment period and low experiment efficiency, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0625C12N2501/999C12N2500/35C12N2500/60
Inventor 王若冰王一鸣
Owner QINGDAO AGRI UNIV
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