Epithelial cell culture solution

Abstract

The invention discloses an epithelial cell culture solution which comprises the following components: 13.4 g / L of DMEM, 5% of fetal calf serum, 10-30mg / L of baicalin, 1-4.5 mg / L of resveratrol, 300-500mg / L of glutathione, 60mg / mL of penicillin and 100mg / L of streptomycin. According to the invention, external stimulation is reduced in the culture process of cells, the proliferation speed of the cells is increased, and the cells meeting the required quantity can be obtained more quickly.

Description

technical field [0001] The invention relates to an epithelial cell culture fluid. Background technique [0002] Cell culture refers to a method of artificially simulating the internal environment in vitro to enable it to survive, grow, reproduce and maintain its main structure and function. Cell culture medium is the material basis for providing nutrition and ensuring the normal growth of cells. The in vitro culture of cells is widely used in the fields of biotechnology and medicine, and is of great significance for the research of human and animal diseases, the development of drugs and even the development of new foods. [0003] At present, the commonly used cell culture medium only adds fetal bovine serum and antibiotics on the basis of DMEM basal culture medium. Some cell lines have poor reproductive ability, poor resistance to environmental changes, and are prone to apoptosis or even necrosis. This results in a long experiment period and low experiment efficiency, whic...

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Problems solved by technology

[0003] At present, the commonly used cell culture medium only adds fetal bovine serum and antibiotics on the basis of DMEM basic culture medium. Some cell lines have poor reproductive ability, poor resistance to environmental changes, and are prone to apoptosis or even necrosis.

It leads to a long experiment cycle and low experiment efficiency, which brings inconvenience to the research based on cell culture in vitro

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