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Yarn for cell culture support, and fabric for cell culture support including same

A cell culture and yarn technology, applied in cell culture supports/coatings, embryonic cells, tissue culture, etc., can solve the problems of inability to cultivate three-dimensional cells, low cell survival rate, inability to cell culture, etc. Cell proliferation, increased proliferation rate, increased adhesion effect

Active Publication Date: 2019-01-04
AMOLIFESCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the scaffolds for cell culture developed so far have a structure similar to that in vivo, and cells cannot be cultured in three dimensions, and the survival rate of cells is not high. appropriate question
[0006] Also, in the process of cell proliferation, since a plurality of cells cultured two-dimensionally or three-dimensionally in a limited space occurs density-dependent inhibition of growth between adjacent cells, there is an inability to Issues with growing cells to target levels

Method used

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  • Yarn for cell culture support, and fabric for cell culture support including same
  • Yarn for cell culture support, and fabric for cell culture support including same
  • Yarn for cell culture support, and fabric for cell culture support including same

Examples

Experimental program
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Effect test

Embodiment approach

[0047] Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art of the present invention can easily implement them. The present invention can be realized in many different forms, and is not limited to the embodiments described here. In order to clarify the present invention, parts irrelevant to the description are omitted from the drawings, and the same reference numerals are assigned to the same or similar structural elements throughout the specification.

[0048] Such as figure 1 As shown, the yarn 10 for the cell culture scaffold according to an embodiment of the present invention comprises a plurality of single yarns 1, 2, a part or all of the twisted yarns that are untwisted and formed by a plurality of single yarns that are separated from each other. Space.

[0049] In the case where the yarn is realized by twisting multiple single yarns in such a way that there is no spa...

Embodiment 1

[0079] A spinning solution was prepared by dissolving PVDF as a fiber-forming component at 15% by weight in DMAc / acetone (Acetone) as a mixed solution. Utilize the electrospinning device to electrospin the spinning solution prepared above, as the electrospinning conditions, the applied voltage is 25KV, the distance between the current collector and the spinning port is 25cm, and the discharge volume is 0.05ml / hole, at RH 65 Under the environment of %30°C, electrospinning was carried out to obtain a nanofiber web roll (Roll) with a width of 1.5m, a weight of 5g / ㎡, and a length of 500m. Figure 7 Part (a) is a photo of the prepared nanofibrous web by winding, Figure 7 Part (b) is a scanning electron micrograph showing nanofibrous webs. Such as Figure 7 As shown in part (b), the average diameter of the nanofibers forming the nanofiber web is about 230 nm.

[0080] Such as Figure 8 As shown in part (a), after slitting the roll of the prepared nanofibrous web with a width of...

experiment example

[0088] A plurality of yarns for cell scaffolds prepared in Examples and Comparative Examples were fixed side by side on a well plate for cell culture. Load 5 × 10 well plates with yarn for cell scaffolds 4 , 2.75×10 5 or 2×10 4 The mesenchymal stem cells (MSCs) were proliferated in DMEM+FBS or KBS-3 basal medium (Basalmedium) at 37°C for 4 days.

[0089] Subsequently, after staining the cultured mesenchymal stem cells (MSCs) with AP or neutral red solution (Neutral red solution), after placing them in the incubator for about 10 minutes, observe the stained cells through an inverted microscope, or add trypsin-EDTA in After being placed in the incubator for about 5 minutes, the number of cells was calculated by a blood counting chamber. Another method is to use a UV-vis spectrometer (UV-vis spectrometer) to measure the absorbance after staining with a cell counting kit 8 (CCK-8). At this time, as a control (control), a cell culture dish (cell culture dish) cultured in 2D und...

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Abstract

Provided is a yarn for a cell culture support. The yarn according to an embodiment of the present invention includes a plurality of twisted monofilament strands, wherein at least a portion of the plurality of twisted monofilament strands are untwisted such that spaces are formed between the monofilament strands in order to prevent density-dependent inhibition of cultured cells and increase a specific surface area for cell contact. Accordingly, as a microenvironment yarn appropriate for the movement, proliferation, and differentiation of cells being cultured is realized, the cell proliferationrate and cell survival rate can be enhanced. Also, as a space for cell proliferation is maximally realized in a limited space in the support, a large amount of cells can be simultaneously cultured, and, because phenomenon of the suppression of cell proliferation due to contact between cells is prevented, cell proliferation can steadily continue. Furthermore, cells cultured through the yarn can becultured in a shape / structure that is more appropriate for being applied to an in vitro experimental model or being implanted in the body of an animal, and can be widely applied in various products used in cell culturing fields or tissue engineering fields such as bioreactors, cell culture containers, or kits for implantation in a body.

Description

technical field [0001] The present invention relates to a yarn for a cell culture scaffold, and more specifically, to a yarn for a cell culture scaffold, a ply yarn containing the same, and a fabric containing the same, that is, a yarn suitable for attachment, migration, proliferation, and differentiation of cultured cells. Microenvironment, thereby improving the survival rate of cells, cells can proliferate three-dimensionally, prevent density-dependent inhibition of contact between cells that proliferate in a limited space due to cell proliferation, and increase the specific surface area that multiple cells can contact . Background technique [0002] Recently, with the expansion of the utilization of cultured cells for disease treatment, interest and research on cell culture are increasing. Cell culture is a technique of taking cells from an organism and culturing them in vitro, differentiating the cultured cells into various body tissues such as skin, organs, nerves, etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D02G3/26D02G1/02D02G3/44C12N5/00A61L27/38A61L27/44
CPCD02G1/02C12N5/0068C12N2513/00C12N2533/30C12N2535/00D02G3/448A61L27/16A61L27/18A61L27/54A61L2300/412A61L27/3804A61L27/3834A61L27/3895A61L27/44C12N5/0062D02G3/26D10B2509/00C12N5/0606C12N5/0618C12N5/0653C12N5/0654C12N5/0658
Inventor 徐寅踊张仙虎具松熙金灿李承勋
Owner AMOLIFESCI CO LTD
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