Full-automatic immunoblotting instrument and detection method

An immunoblotting, fully automatic technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of long moving tracks, large space occupation, large space, etc., to achieve accurate test data, reduce space occupation, and scientific test results. Effect

Pending Publication Date: 2020-10-30
欧亚先锋生物技术投资(北京)有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The above-mentioned prior art solution has the following defects: the film strip grooves are arranged along the length direction of the body, which takes up a large space, and the long moving track is set, so that the whole equipment has a large volume and takes up a large space

Method used

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  • Full-automatic immunoblotting instrument and detection method
  • Full-automatic immunoblotting instrument and detection method
  • Full-automatic immunoblotting instrument and detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0071] Embodiment 1: a kind of automatic immunoblotting instrument, such as figure 1 As shown, it includes a frame 1. The frame 1 includes a bottom plate 1.2 and a first side plate 1.1. The first side plate 1.1 is vertically fixed on the surface of the bottom plate 1.2. One side of the first side plate 1.1 is integrally formed with a first bearing platform 1.3 It is parallel to the second loading platform 1.4, the first loading platform 1.3, the second loading platform 1.4 and the bottom plate 1.2, and the setting height of the first loading platform 1.3 is lower than that of the second loading platform 1.4.

[0072] Such as Figure 4 with Figure 5 As shown, the first carrying platform 1.3 is rotatably provided with a large plate 2.1. The shape of the large plate 2.1 is disc-shaped, and it is arranged parallel to the first carrying platform 1.3. The bottom plate 1.2 is provided with a drive plate 2.1 to rotate on the surface of the first carrying platform 1.3 The first driv...

Embodiment 2

[0119] Embodiment 2: A kind of automatic immunoblotting detection method, utilizes embodiment 1 to carry out immunoblotting detection, control steps are as follows:

[0120] Step 1: Before testing, install multiple kits on the small plate 9, the kits are divided into cleaning solution kit 11.1, substrate solution kit 11.2, stop solution kit 11.3, diluent kit 11.4, distilled water reagent Box 11.5 and CON binding solution kit 11.6, the reaction vessel 6 and sample box 8.2 are installed on the large plate 2.1, the CON reagent refers to the binding solution reagent, and in the present invention, the binding solution refers to the use of horseradish peroxidase to mark the antibody Binding of human IgG monoclonal antibody to antibody;

[0121] Step 2: The large plate 2.1 is rotated, and the reaction vessel 6 is rotated to the reagent filling station below the reagent filling device 13. At the reagent filling station, the reaction tank 6.3, the through hole 9.1, and the gap 9.2 on t...

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Abstract

The invention relates to a full-automatic immunoblotting instrument and a detection method. The full-automatic immunoblotting instrument comprises a rack, wherein kits, a reagent filling device, reaction vessels with membrane strips, sample boxes, and a sample filling mechanism used for adding sample liquid in the sample box into the reaction vessel are arranged on the rack. A large disc and a small disc which are coaxial are rotationally arranged on the rack; the plurality of kits are arranged on the small disc along the circumferential direction of the small disc; the reagent filling deviceis positioned at the outer edge of the small disc; a plurality of reaction vessels and a plurality of sample boxes are arranged in the circumferential direction of the large disc; the sample boxes arearranged on the edge of the large disc; the reaction vessels are installed between the center of the large disc and the sample boxes, and the sample filling mechanism is arranged on a short-stroke rail and moves in a radial direction of the large disc. The space structure of the large disc and the small disc is reasonably and efficiently utilized, the whole instrument tends to be miniaturized, efficient and automatic, a disposable suction nozzle is clamped, and cross infection is avoided.

Description

technical field [0001] The invention relates to the technical field of automatic immunoblotting detection, in particular to a fully automatic immunoblotting instrument and a detection method. Background technique [0002] Western blotting is widely used in autoantibody detection, allergen detection, and protein detection. It is mainly for qualitative and semi-quantitative analysis of the detected substances. In order to realize the efficient implementation of this method, a variety of fully automatic Western blotting instruments, and most western blotting instruments on the market, have the problem of low experimental efficiency. [0003] In order to solve the above problems, in a Chinese patent document with the authorized announcement number CN207992247U, a fully automatic immunoblotting instrument is described, including a body, a moving track, a film strip groove and a sample rack are arranged on the body, and the film strip groove is used For accommodating the imprinti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N35/02G01N35/04G01N35/10G01N33/53
CPCG01N33/5302G01N35/00G01N35/00584G01N35/025G01N35/04G01N35/1009G01N2035/00089G01N2035/00465G01N2035/0439
Inventor 李东琦
Owner 欧亚先锋生物技术投资(北京)有限责任公司
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