Chicken semen diluent capable of obtaining high fertilization rate, preparation method and use method thereof

Abstract

The invention discloses a chicken semen diluent capable of obtaining a high fertilization rate, wherein every 1 L of the semen diluent comprises the following components by weight: 14-16 g of glucose;3.3-3.6 g of potassium dihydrogen phosphate; 22-26 g of disodium hydrogen phosphate; 13-15 g of sodium glutamate; 0.5 to 1.5 g of disodium ethylene diamine tetraacetate; 80000-120000 units of penicillin; and 80-120 mg of streptomycin. The preparation and use method comprises the following steps: 1) putting the components into a beaker, adding a proper amount of distilled water, fully dissolving,transferring into a 1 L volumetric flask, and fixing the volume to 1 L to prepare a chicken semen diluent; 2) quantitatively subpackaging the chicken semen diluent, and carrying out short-term preservation; 3) preheating the chicken semen diluent; 4) diluting the semen; and 5) sucking the semen by a semen deposition gun or a dropper, and inputting the semen into a hen fallopian tube. According tothe invention, the number of matched hens is increased, the cock feeding amount is reduced, and through low-temperature preservation, sperm metabolism is reduced.

Description

technical field [0001] The invention relates to the field of poultry breeding, in particular to a diluent of chicken semen capable of obtaining a high fertilization rate and a preparation and use method thereof. Background technique [0002] With the development of animal husbandry, the breeding method of breeders in my country is gradually changing from the flat raising method to the cage raising method. The cage raising method has the characteristics of small footprint and easy management, which is especially suitable for my country's per capita land resources and low breeding level. However, artificial insemination is an indispensable breeding technology for caged breeders. Through investigation, it is found that most of the breeding chicken production units currently use raw semen for artificial insemination. The semen collection volume of breeding roosters is 0.2-0.9 mL at a time, the average semen collection volume is 0.4 mL, and the sperm density of semen is 3-4 billio...

AI Technical Summary

Problems solved by technology

Through investigation, it is found that most of the breeding chicken production units currently use raw semen for artificial insemination. The semen collection volume of breeding roosters is 0.2-0.9 mL at a time, the average semen collection volume is 0.4 mL, and the sperm density of semen is 3-4 billion / mL , studies have shown that the number of effective sperms in one insemination of a hen reaches 0.8-100 million, and a higher insemination rate can be obtained. In production, each hen is generally transfused with 0.03 mL of original semen, and a rooster can only be fertilized with 13- About 15, this will undoubtedly increase the number of roosters and increase the production cost of breeders. At the same time, the storage time of the original semen at room temperature is limited, generally not more than 0.5 h, otherwise the sperm survival rate, vitality, deformity The rate will increase significantly, thereby reducing the quality of semen and affecting the fertilization rate of eggs

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