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Asmt expression promoter

A technology for expressing accelerators and accelerators, which can be applied in the directions of anti-inflammatory agents, non-central analgesics, preparations for skin care, etc. Function improvement, enhancement and improvement effect

Pending Publication Date: 2020-11-13
SHISEIDO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, it has not been fully elucidated which substance greatly contributes to the ASMT expression-promoting effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Enhancement of ASMT gene expression by ectoine

[0066] Sample preparation

[0067] Commercially available ectoine (Melque) was dissolved in purified water and filtered to prepare an ectoine sample with a concentration of 1M.

[0068] Culture of epidermal keratinocytes

[0069] Commercially available keratinocytes (クラボウ) derived from normal adult skin were made into 5×10 5 Cells / flasks were inoculated in T75 flasks, and cultured in keratinocyte culture medium (Humedia-KG2, クラボウ) at 37°C, 5% CO 2 Culture was carried out under atmosphere until reaching semiconfluency, as seeding cells. The keratinocytes were recovered by trypsinization to become 2×10 4 The cells / well were seeded in 24-well plates, and the culture medium for keratinocytes (Humedia-KG2, Kurabou) was incubated at 37°C and 5% CO. 2 Incubation was carried out under atmosphere until confluency was reached.

[0070] Addition of samples

[0071] To confluent epidermal keratinocytes, an ectoine sample was a...

Embodiment 2

[0078] Concentration dependence of ectoine against ASMT gene expression

[0079] Sample preparation

[0080] A 10% by weight ectoine sample was prepared by dissolving commercially available ectoine (Melque) in purified water and filter-sterilizing it.

[0081] Addition of samples

[0082] In the epidermal keratinocytes cultured in the same manner as in Example 1, ectoine samples were added so that the concentration in the medium was 0.005% by weight, 0.01% by weight, 0.02% by weight, 0.05% by weight, and 0.1% by weight. Add at appropriate dilution rate, at 37°C, 5% CO 2 Continue to cultivate under the atmosphere.

[0083] RNA extraction

[0084] Ten hours after the addition of ectoine, the medium was removed, and the cells were lysed and RNA was extracted using a commercially available RNA extraction reagent (RNeasyMini Kit, Qiagen).

[0085] Evaluation of ASMT expression by quantitative PCR

[0086] Using the extracted RNA as a template, quantitative PCR was performed u...

Embodiment 3

[0089] Enhancement of ASMT gene expression by ectoine compared with various antioxidant substances

[0090] Sample preparation

[0091] An ectoine sample was prepared by the same method as in Example 1. As a control drug, samples containing substances known to have an antioxidant effect (glucosyl hesperidin, green tea extract, oolong tea extract) were also produced. Commercially available glucosyl hesperidin (Toyo Seisano) was dissolved in purified water and filtered to prepare a 100 mM glucosyl hesperidin sample. Green tea extract (Karei Kogyo) and oolong tea extract (Santory) were used after filter-sterilizing commercially available extracts.

[0092] In the epidermal keratinocytes cultured in the same manner as in Example 1, the concentrations of each sample obtained above were ectoine 1 mM, glucosyl hesperidin 100 μM, and green tea extract 0.1 μM in the culture medium. %, 0.1% of oolong tea extract was added to the culture medium at an appropriate dilution rate. These ...

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PUM

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Abstract

Provided is a novel ASMT expression promoter. The present invention provides an ASMT expression promoter that contains ectoine or a physiologically acceptable salt thereof as an active ingredient. Thepresent invention also provides a melatonin synthesis promoter that contains ectoine or a physiologically acceptable salt thereof as an active ingredient and that promotes melatonin synthesis via promotion of ASMT expression. The outcomes of improvements in sleep disorders, enhanced cognitive function, improvements in mood disorders, enhanced antioxidation activity, and enhanced antiinflammatoryactivity can be strengthened by promoting melatonin synthesis via promotion of ASMT gene expression.

Description

technical field [0001] The present invention relates to ASMT expression promoters. Background technique [0002] Melatonin is a hormone present in animals and plants, and it has been reported that there are effects such as improvement of sleep disturbance, improvement of cognitive function, improvement of mood disorder, and enhancement of antioxidant activity / anti-inflammatory activity (Patent Documents 1, 2, Non- Patent Document 1). [0003] Acetyl serotonin O-methyltransferase (ASMT) is known as a substance that promotes synthesis of melatonin. ASMT is an enzyme that is produced in vivo through the expression of the ASMT gene and participates in the final step of the melatonin synthesis pathway. Therefore, it is considered that the synthesis of melatonin is promoted by promoting the expression of ASMT, and the amount of melatonin increases (Patent Document 2, Non-Patent Documents 1 to 6). [0004] Therefore, it is desired to search for a substance that promotes the synt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/505A61K8/49A61P25/00A61P29/00A61Q19/00
CPCA61P29/00A61Q19/00A61K31/505A61P25/00A23L29/035A61K8/4953A61K2800/10A23L33/10
Inventor 合津阳子土师信一郎
Owner SHISEIDO CO LTD
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